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Literature summary extracted from

  • Buan, N.R.; Suh, S.J.; Escalante-Semerena, J.C.
    The eutT gene of Salmonella enterica encodes an oxygen-labile, metal-containing ATP:corrinoid adenosyltransferase enzyme (2004), J. Bacteriol., 186, 5708-5714.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.17 coenzyme B12 i.e. adenosylcob(III)alamin, is required for stimulation of eut operon expression Salmonella enterica
2.5.1.17 coenzyme B12 i.e. adenosylcob(III)alamin, is required for stimulation of eut operon expression Salmonella enterica subsp. enterica serovar Typhimurium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.17 2 enzymes encoded by genes eutT and cobA, DNA sequence determination, analysis of several geno- and phenotypic strains, enzyme is expressed e.g. as fusion protein or to complement a deficient strain, overview Salmonella enterica
2.5.1.17 2 enzymes encoded by genes eutT and cobA, DNA sequence determination, analysis of several geno- and phenotypic strains, enzyme is expressed e.g. as fusion protein or to complement a deficient strain, overview Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.17 bathophenanthroline inhibition of EutT Salmonella enterica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.17 Fe2+ required and contained by EutT Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.17 ATP + cob(I)alamin Salmonella enterica last step in the corrinoid adenosylation pathway, EutT and CobA triphosphate + adenosylcob(III)alamin
-
ir
2.5.1.17 ATP + cob(I)alamin Salmonella enterica subsp. enterica serovar Typhimurium last step in the corrinoid adenosylation pathway, EutT and CobA triphosphate + adenosylcob(III)alamin
-
ir
2.5.1.17 additional information Salmonella enterica EutT is necessary and sufficient for growth of a cobA eutT strain on ethanolamine as a carbon and energy or nitrogen source ?
-
?
2.5.1.17 additional information Salmonella enterica subsp. enterica serovar Typhimurium EutT is necessary and sufficient for growth of a cobA eutT strain on ethanolamine as a carbon and energy or nitrogen source ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.17 Salmonella enterica
-
EutT; 2 enzymes encoded by genes eutT and cobA with different primary sequences, cobA is a housekeeping gene, several derivatives of strain TR6583, several geno- and phenotypes, overview
-
2.5.1.17 Salmonella enterica subsp. enterica serovar Typhimurium P31570 CobA; 2 enzymes encoded by genes eutT and cobA with different primary sequences, cobA is a housekeeping gene, several derivatives of strain TR6583, several geno- and phenotypes, overview
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.5.1.17 EutT is oxygen-labile, exposure to air leads to complete loss of activity Salmonella enterica
2.5.1.17 EutT is oxygen-labile, exposure to air leads to complete loss of activity Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.17 0.0061
-
strain carrying the complementation plasmd eutT+, in vivo activity Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.17 0.0061
-
strain carrying the complementation plasmid eutT+, in vivo activity Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.17 ATP + cob(I)alamin last step in the corrinoid adenosylation pathway, EutT and CobA Salmonella enterica triphosphate + adenosylcob(III)alamin
-
ir
2.5.1.17 ATP + cob(I)alamin last step in the corrinoid adenosylation pathway, EutT and CobA Salmonella enterica subsp. enterica serovar Typhimurium triphosphate + adenosylcob(III)alamin
-
ir
2.5.1.17 ATP + cob(I)alamin CobA has an ATP-binding P-loop motif, while EutT has a cysteine-rich region reminiscent of a conserved S-adenosylmethionine Fe-S cluster motif Salmonella enterica triphosphate + adenosylcob(III)alamin
-
ir
2.5.1.17 ATP + cob(I)alamin CobA has an ATP-binding P-loop motif, while EutT has a cysteine-rich region reminiscent of a conserved S-adenosylmethionine Fe-S cluster motif Salmonella enterica subsp. enterica serovar Typhimurium triphosphate + adenosylcob(III)alamin
-
ir
2.5.1.17 additional information EutT is necessary and sufficient for growth of a cobA eutT strain on ethanolamine as a carbon and energy or nitrogen source Salmonella enterica ?
-
?
2.5.1.17 additional information EutT is necessary and sufficient for growth of a cobA eutT strain on ethanolamine as a carbon and energy or nitrogen source Salmonella enterica subsp. enterica serovar Typhimurium ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.17 ATP:co(I)rrinoid adenosyltransferase
-
Salmonella enterica
2.5.1.17 ATP:co(I)rrinoid adenosyltransferase
-
Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.17 ATP:corrinoid adenosyltransferase
-
Salmonella enterica
2.5.1.17 ATP:corrinoid adenosyltransferase
-
Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.17 CobA
-
Salmonella enterica
2.5.1.17 CobA
-
Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.17 cobalamin adenosyltransferase
-
Salmonella enterica
2.5.1.17 cobalamin adenosyltransferase
-
Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.17 EutT
-
Salmonella enterica
2.5.1.17 EutT
-
Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.17 25
-
assay at Salmonella enterica
2.5.1.17 25
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.17 8
-
assay at Salmonella enterica
2.5.1.17 8
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium