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Literature summary extracted from

  • Kitagawa, W.; Kimura, N.; Kamagata, Y.
    A novel p-nitrophenol degradation gene cluster from a gram-positive bacterium, Rhodococcus opacus SAO101 (2004), J. Bacteriol., 186, 4894-4902.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.37 expression in Escherichia coli Rhodococcus opacus
1.14.13.167 expressed in Escherichia coli Rosetta(DE3)pLysS cells Rhodococcus opacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.37 33200
-
 x * 33200, deduced from nucleotide sequence Rhodococcus opacus
1.14.13.167 59700
-
 x * 59700, calculated from amino acid sequence Rhodococcus opacus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.37 Rhodococcus opacus
-
-
-
1.13.11.37 Rhodococcus opacus SAO101
-
-
-
1.14.13.167 Rhodococcus opacus
-
-
-
1.14.13.167 Rhodococcus opacus SAO101
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.167 culture condition:4-nitrophenol-grown cell
-
 Rhodococcus opacus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.167 0.0000046
-
 using 2,4,6-trinitrocatechol as substrate, pH and temperature not specified in the publication Rhodococcus opacus
1.14.13.167 0.000025
-
 using 4-nitrocatechol as substrate, pH and temperature not specified in the publication Rhodococcus opacus
1.14.13.167 0.0000264
-
 using 4-nitrophenol as substrate, pH and temperature not specified in the publication Rhodococcus opacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.37 benzene-1,2,4-triol + O2
-
 Rhodococcus opacus 3-hydroxy-cis,cis-muconate
-
 ?
1.13.11.37 benzene-1,2,4-triol + O2
-
 Rhodococcus opacus SAO101 3-hydroxy-cis,cis-muconate
-
 ?
1.14.13.167 2,4,6-trinitrophenol + NADH + H+ + O2 17% activity compared to 4-nitrophenol Rhodococcus opacus ?
-
 ?
1.14.13.167 2,4,6-trinitrophenol + NADH + H+ + O2 17% activity compared to 4-nitrophenol Rhodococcus opacus SAO101 ?
-
 ?
1.14.13.167 4-nitrocatechol + NADH + H+ + O2 96% activity compared to 4-nitrophenol Rhodococcus opacus 1,2,4-trihydroxybenzene + nitrite + NAD+ + H2O
-
 ?
1.14.13.167 4-nitrocatechol + NADH + H+ + O2 96% activity compared to 4-nitrophenol Rhodococcus opacus SAO101 1,2,4-trihydroxybenzene + nitrite + NAD+ + H2O
-
 ?
1.14.13.167 4-nitrophenol + NADH + H+ + O2 100% activity Rhodococcus opacus ?
-
 ?
1.14.13.167 4-nitrophenol + NADH + H+ + O2 100% activity Rhodococcus opacus SAO101 ?
-
 ?
1.14.13.167 additional information almost no activity towards 2,4,6-trinitrophenol Rhodococcus opacus ?
-
 ?
1.14.13.167 additional information almost no activity towards 2,4,6-trinitrophenol Rhodococcus opacus SAO101 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.37 ? x * 33200, deduced from nucleotide sequence Rhodococcus opacus
1.14.13.167 ? x * 59700, calculated from amino acid sequence Rhodococcus opacus

Synonyms

EC Number Synonyms Comment Organism
1.13.11.37 NpcC
-
 Rhodococcus opacus
1.14.13.167 4-NP/4-NCA monooxygenase
-
 Rhodococcus opacus
1.14.13.167 NpcA
-
 Rhodococcus opacus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.167 FAD
-
 Rhodococcus opacus

General Information

EC Number General Information Comment Organism
1.14.13.167 malfunction the npcA mutant strain SDA1 completely loses the ability to grow on 4-nitrophenol as the sole carbon source Rhodococcus opacus