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Literature summary extracted from

  • Brinsmade, S.R.; Escalante-Semerena, J.C.
    The eutD gene of Salmonella enterica encodes a protein with phosphotransacetylase enzyme activity (2004), J. Bacteriol., 186, 1890-1892.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.8 expressed as His-tag fusion protein in Escherichia coli BL21(lambdaDE3) Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.8 0.046
-
 CoA 30°C Salmonella enterica
2.3.1.8 0.129
-
 acetyl phosphate 30°C Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate Salmonella enterica enables growth on acetate as carbon and energy source, required for ethanolamine catabolism CoA + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.8 Salmonella enterica
-
 serovar typhimurium LT2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.8 recombinant protein using His-tag Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate
-
 Salmonella enterica CoA + acetyl phosphate
-
 r
2.3.1.8 acetyl-CoA + phosphate enables growth on acetate as carbon and energy source, required for ethanolamine catabolism Salmonella enterica CoA + acetyl phosphate
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.8 25
-
 50% loss of activity within 5 min Salmonella enterica
2.3.1.8 45
-
 90% loss of activity within 5 min Salmonella enterica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.8 1927
-
 acetyl phosphate 30°C, calculated per monomer Salmonella enterica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.8 7.2
-
-
 Salmonella enterica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.8 6.5 7.8 90% loss of activity at pH 6.5, 25% loss of activity at pH 7.8 Salmonella enterica