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Literature summary extracted from

  • Ge, Y.; Eltis, L.D.
    Characterization of hybrid toluate and benzoate dioxygenases (2003), J. Bacteriol., 185, 5333-5341.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.10 expressed in Pseudomonas putida CL01 as His-tag fusion protein Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.10 0.026
-
 benzoate pH 7, 25°C Acinetobacter calcoaceticus
1.14.12.10 0.089
-
 3-methylbenzoate pH 7, 25°C Acinetobacter calcoaceticus
1.14.12.10 0.113
-
 3-chlorobenzoate pH 7, 25°C Acinetobacter calcoaceticus
1.14.12.10 1.401
-
 2-Methylbenzoate pH 7, 25°C Acinetobacter calcoaceticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.10 Iron active site mononuclear iron Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.10 benzoate + O2 + NADH Acinetobacter calcoaceticus catabolism of aromatic compounds 2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 benzoate + O2 + NADH Acinetobacter calcoaceticus BADO ADP1 catabolism of aromatic compounds 2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.10 Acinetobacter calcoaceticus
-
-
-
1.14.12.10 Acinetobacter calcoaceticus BADO ADP1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.10 recombinant and native enzyme Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.10 5
-
 pH 7, 25°C, with 3-methylbenzoate as substrate Acinetobacter calcoaceticus

Storage Stability

EC Number Storage Stability Organism
1.14.12.10 -80°C, stable for at least 6 months Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.10 2-methylbenzoate + O2 + NADH
-
 Acinetobacter calcoaceticus cis-1,2-dihydro-1,2-dihydroxy-2-methyl-benzoate + NAD+
-
 ?
1.14.12.10 2-methylbenzoate + O2 + NADH
-
 Acinetobacter calcoaceticus BADO ADP1 cis-1,2-dihydro-1,2-dihydroxy-2-methyl-benzoate + NAD+
-
 ?
1.14.12.10 3-chlorobenzoate + O2 + NADH + H+
-
 Acinetobacter calcoaceticus 3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 3-chlorobenzoate + O2 + NADH + H+
-
 Acinetobacter calcoaceticus BADO ADP1 3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 3-methylbenzoate + O2 + NADH + H+
-
 Acinetobacter calcoaceticus 3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 3-methylbenzoate + O2 + NADH + H+
-
 Acinetobacter calcoaceticus BADO ADP1 3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 benzoate + O2 + NADH
-
 Acinetobacter calcoaceticus 2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 benzoate + O2 + NADH catabolism of aromatic compounds Acinetobacter calcoaceticus 2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 benzoate + O2 + NADH
-
 Acinetobacter calcoaceticus BADO ADP1 2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 benzoate + O2 + NADH catabolism of aromatic compounds Acinetobacter calcoaceticus BADO ADP1 2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.12.10 3
-
 3-chlorobenzoate pH 7, 25°C Acinetobacter calcoaceticus
1.14.12.10 4.2
-
 2-Methylbenzoate pH 7, 25°C Acinetobacter calcoaceticus
1.14.12.10 7
-
 3-methylbenzoate pH 7, 25°C Acinetobacter calcoaceticus
1.14.12.10 8.6
-
 benzoate pH 7, 25°C Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.10 iron-sulfur centre [2Fe-2S] Rieske center Acinetobacter calcoaceticus
1.14.12.10 NADH
-
 Acinetobacter calcoaceticus