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Literature summary extracted from

  • Wolterink, A.F.; Schiltz, E.; Hagedoorn, P.L.; Hagen, W.R.; Kengen, S.W.; Stams, A.J.
    Characterization of the chlorate reductase from Pseudomonas chloritidismutans (2003), J. Bacteriol., 185, 3210-3213.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.97.1.1 0.159
-
 Chlorate pH 7.0, 30°C Stutzerimonas chloritidismutans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.97.1.1 cytoplasm
-
 Stutzerimonas chloritidismutans 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.97.1.1 Iron nonheme iron Stutzerimonas chloritidismutans
1.97.1.1 Molybdenum bound to enzyme Stutzerimonas chloritidismutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.97.1.1 34000
-
 alphabetagamma, 1 * 97000 + 1 * 38000 + 1 * 34000, SDS-PAGE, native mass by gel filtration Stutzerimonas chloritidismutans
1.97.1.1 38000
-
 alphabetagamma, 1 * 97000 + 1 * 38000 + 1 * 34000, SDS-PAGE, native mass by gel filtration Stutzerimonas chloritidismutans
1.97.1.1 97000
-
 alphabetagamma, 1 * 97000 + 1 * 38000 + 1 * 34000, SDS-PAGE, native mass by gel filtration Stutzerimonas chloritidismutans
1.97.1.1 167000
-
 gel filtration Stutzerimonas chloritidismutans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.97.1.1 chlorate + reduced electron acceptor Stutzerimonas chloritidismutans dissimilatory chlorate reduction, chlorate serves as terminal electron acceptor chlorite + H2O + oxidized electron acceptor
-
 ?
1.97.1.1 chlorate + reduced electron acceptor Stutzerimonas chloritidismutans AW-1 / DSM 13592 dissimilatory chlorate reduction, chlorate serves as terminal electron acceptor chlorite + H2O + oxidized electron acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.97.1.1 Stutzerimonas chloritidismutans P83448 N-terminal sequence of the alpha subunit
-
1.97.1.1 Stutzerimonas chloritidismutans P83449 N-terminal sequence of the beta subunit
-
1.97.1.1 Stutzerimonas chloritidismutans P83450 N-terminal sequence of the gamma subunit
-
1.97.1.1 Stutzerimonas chloritidismutans AW-1 / DSM 13592 P83448 N-terminal sequence of the alpha subunit
-
1.97.1.1 Stutzerimonas chloritidismutans AW-1 / DSM 13592 P83449 N-terminal sequence of the beta subunit
-
1.97.1.1 Stutzerimonas chloritidismutans AW-1 / DSM 13592 P83450 N-terminal sequence of the gamma subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.97.1.1
-
 Stutzerimonas chloritidismutans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.97.1.1 42
-
 purified enzyme, pH 7.0, 30°C Stutzerimonas chloritidismutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.97.1.1 bromate + reduced methyl viologen
-
 Stutzerimonas chloritidismutans bromite + H2O + methyl viologen
-
 ?
1.97.1.1 bromate + reduced methyl viologen
-
 Stutzerimonas chloritidismutans AW-1 / DSM 13592 bromite + H2O + methyl viologen
-
 ?
1.97.1.1 chlorate + reduced electron acceptor dissimilatory chlorate reduction, chlorate serves as terminal electron acceptor Stutzerimonas chloritidismutans chlorite + H2O + oxidized electron acceptor
-
 ?
1.97.1.1 chlorate + reduced electron acceptor dissimilatory chlorate reduction, chlorate serves as terminal electron acceptor Stutzerimonas chloritidismutans AW-1 / DSM 13592 chlorite + H2O + oxidized electron acceptor
-
 ?
1.97.1.1 chlorate + reduced methyl viologen
-
 Stutzerimonas chloritidismutans chlorite + H2O + methyl viologen
-
 ?
1.97.1.1 chlorate + reduced methyl viologen
-
 Stutzerimonas chloritidismutans AW-1 / DSM 13592 chlorite + H2O + methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.97.1.1 trimer alphabetagamma, 1 * 97000 + 1 * 38000 + 1 * 34000, SDS-PAGE, native mass by gel filtration Stutzerimonas chloritidismutans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.97.1.1 75
-
-
 Stutzerimonas chloritidismutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.97.1.1 7.5
-
-
 Stutzerimonas chloritidismutans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.97.1.1 5.5 10 50% of maximum activity at pH 5.5 and 10 Stutzerimonas chloritidismutans