Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • McKay, D.B.; Prucha, M.; Reineke, W.; Timmis, K.N.; Pieper, D.H.
    Substrate specificity and expression of three 2,3-dihydroxybiphenyl 1,2-dioxygenases from Rhodococcus globerulus strain P6 (2003), J. Bacteriol., 185, 2944-2951.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.39 0.00045
-
 3'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.001
-
 2,3-Dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.001
-
 2'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.0015
-
 4'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.01
-
 3'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.011
-
 4'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.012
-
 4'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.018
-
 2'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.019
-
 2'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.026
-
 2,3-Dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.029
-
 2,3-Dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.1
-
 3-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 1.23
-
 3-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 1.94
-
 3-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 2.4
-
 catechol 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 2.8
-
 4-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 61
-
 4-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 93
-
 4-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.39 Fe2+
-
 Rhodococcus globerulus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.39 28000
-
 x * 28000, SDS-PAGE Rhodococcus globerulus
1.13.11.39 29000
-
 x * 29000, SDS-PAGE Rhodococcus globerulus
1.13.11.39 34000
-
 x * 34000, SDS-PAGE Rhodococcus globerulus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.39 Rhodococcus globerulus
-
-
-
1.13.11.39 Rhodococcus globerulus P6
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.13.11.39 50% loss of activity after 4.5 min in air-satured buffer Rhodococcus globerulus
1.13.11.39 50% loss of activity after 43 min in air-satured buffer Rhodococcus globerulus
1.13.11.39 50% loss of activity after 7.2 min in air-satured buffer Rhodococcus globerulus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.39
-
 Rhodococcus globerulus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.39 7.5
-
-
 Rhodococcus globerulus
1.13.11.39 20
-
-
 Rhodococcus globerulus
1.13.11.39 30
-
-
 Rhodococcus globerulus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.39 2',3',5'-trichloro-2,3-dihydroxybiphenyl + O2 49% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(2',3',5'-trichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 2',3',5'-trichloro-2,3-dihydroxybiphenyl + O2 49% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus P6 2-hydroxy-6-oxo-6-(2',3',5'-trichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 2',3'-dichloro-2,3-dihydroxybiphenyl + O2 25% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(2',3'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 2',3'-dichloro-2,3-dihydroxybiphenyl + O2 57% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(2',3'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 2',3'-dichloro-2,3-dihydroxybiphenyl + O2 25% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus P6 2-hydroxy-6-oxo-6-(2',3'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 2',3'-dichloro-2,3-dihydroxybiphenyl + O2 57% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus P6 2-hydroxy-6-oxo-6-(2',3'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 2',4'-dichloro-2,3-dihydroxybiphenyl + O2 33% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(2',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 2',4'-dichloro-2,3-dihydroxybiphenyl + O2 63% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(2',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 2'-chloro-2,3-dihydroxybiphenyl + O2
-
 Rhodococcus globerulus 2-hydroxy-6-oxo-6-(2'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 2'-chloro-2,3-dihydroxybiphenyl + O2 50% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(2'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 2,3-dihydroxybiphenyl + O2
-
 Rhodococcus globerulus 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 3',4',5'-trichloro-2,3-dihydroxybiphenyl + O2 11% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',4',5'-trichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 3',4',5'-trichloro-2,3-dihydroxybiphenyl + O2 26% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',4',5'-trichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 3',4'-dichloro-2,3-dihydroxybiphenyl + O2 49% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 3',4'-dichloro-2,3-dihydroxybiphenyl + O2 59% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 3',4'-dichloro-2,3-dihydroxybiphenyl + O2 61% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 3',5'-dichloro-2,3-dihydroxybiphenyl + O2 15% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',5'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 3',5'-dichloro-2,3-dihydroxybiphenyl + O2 49% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',5'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 3',5'-dichloro-2,3-dihydroxybiphenyl + O2 61% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',5'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 3'-chloro-2,3-dihydroxybiphenyl + O2
-
 Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 3'-chloro-2,3-dihydroxybiphenyl + O2 64% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 3'-chloro-2,3-dihydroxybiphenyl + O2 71% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 3'-chloro-2,3-dihydroxybiphenyl + O2 95% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 3-methylcatechol + O2
-
 Rhodococcus globerulus ?
-
 ?
1.13.11.39 3-methylcatechol + O2
-
 Rhodococcus globerulus P6 ?
-
 ?
1.13.11.39 4'-chloro-2,3-dihydroxybiphenyl + O2
-
 Rhodococcus globerulus 2-hydroxy-6-oxo-6-(4'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 4'-chloro-2,3-dihydroxybiphenyl + O2 61% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(4'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 4'-chloro-2,3-dihydroxybiphenyl + O2 77% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(4'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 4'-chloro-2,3-dihydroxybiphenyl + O2 84% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(4'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 4-methylcatechol + O2
-
 Rhodococcus globerulus ?
-
 ?
1.13.11.39 catechol + O2
-
 Rhodococcus globerulus ?
-
 ?
1.13.11.39 catechol + O2
-
 Rhodococcus globerulus P6 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.39 ? x * 28000, SDS-PAGE Rhodococcus globerulus
1.13.11.39 ? x * 34000, SDS-PAGE Rhodococcus globerulus
1.13.11.39 ? x * 29000, SDS-PAGE Rhodococcus globerulus

Synonyms

EC Number Synonyms Comment Organism
1.13.11.39 BphC1
-
 Rhodococcus globerulus
1.13.11.39 BphC2
-
 Rhodococcus globerulus
1.13.11.39 BphC3
-
 Rhodococcus globerulus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.39 0.016
-
 catechol 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.064
-
 4-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.24
-
 4-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.25
-
 2'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.36
-
 4-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 0.92
-
 catechol 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 3.5
-
 2'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 4
-
 3'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 4.4
-
 3-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 4.5
-
 4'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 4.8
-
 2,3-Dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 7.5
-
 2'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 8.3
-
 4'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 8.5
-
 3'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 9.8
-
 3-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 9.8
-
 2,3-Dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 12
-
 3'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 13.6
-
 3-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 16.5
-
 2,3-Dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
1.13.11.39 18.8
-
 4'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus