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Literature summary extracted from

  • Diaz, E.; Ferrandez, A.; Garcia, J.L.
    Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12 (1998), J. Bacteriol., 180, 2915-2923.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.87 expressed in Escherichia coli BL21(DE3) cells Escherichia coli
1.14.12.19 expressed in Salmonella typhimurium Escherichia coli
3.7.1.14
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.87 28500
-
 calculated from amino acid sequence Escherichia coli
1.14.12.19 11328
-
 gene products from hcaA1, hcaA2, hcaC and hcaD, 1 * 51109 + 1 * 20579 + 1 * 11328 + 1 * 43978, subunit masses calculated from deduced amino acid sequences Escherichia coli
1.14.12.19 20579
-
 gene products from hcaA1, hcaA2, hcaC and hcaD, 1 * 51109 + 1 * 20579 + 1 * 11328 + 1 * 43978, subunit masses calculated from deduced amino acid sequences Escherichia coli
1.14.12.19 43978
-
 gene products from hcaA1, hcaA2, hcaC and hcaD, 1 * 51109 + 1 * 20579 + 1 * 11328 + 1 * 43978, subunit masses calculated from deduced amino acid sequences Escherichia coli
1.14.12.19 51109
-
 gene products from hcaA1, hcaA2, hcaC and hcaD, 1 * 51109 + 1 * 20579 + 1 * 11328 + 1 * 43978, subunit masses calculated from deduced amino acid sequences Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.19 3-phenylpropionate + NADH + H+ + O2 Escherichia coli enables utilization of 3-phenylpropionate as carbon source, important role in wine making, aging and storage 3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dien-1-yl]propanoic acid + NAD+
-
 ?
3.7.1.14 (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O Escherichia coli the enzyme is involved in 3-phenylpropanoate catabolism (2E)-2-hydroxypenta-2,4-dienoate + succinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.87 Escherichia coli
-
-
-
1.14.12.19 Escherichia coli
-
-
-
1.14.13.127 Escherichia coli
-
-
-
3.7.1.14 Escherichia coli P0ABW0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.87 Ni-NTA column chromatography and Sephadex G-25M gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.87 3-phenylpropionate + NAD+
-
 Escherichia coli 3-(2,3-dihydroxyphenyl) propionate + NADH + H+
-
 ?
1.3.1.87 cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + NAD+
-
 Escherichia coli ? + NADH + H+
-
 ir
1.3.1.87 cis-3-(2-carboxyethyl)-3,5-cyclohexadien-1,2-diol + NAD+
-
 Escherichia coli 3-(2,3-dihydroxyphenyl)propionate + NADH + H+
-
 ir
1.14.12.19 3-phenylpropionate + NADH + H+ + O2
-
 Escherichia coli 3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dien-1-yl]propanoic acid + NAD+
-
 ?
1.14.12.19 3-phenylpropionate + NADH + H+ + O2 enables utilization of 3-phenylpropionate as carbon source, important role in wine making, aging and storage Escherichia coli 3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dien-1-yl]propanoic acid + NAD+
-
 ?
1.14.12.19 cinnamic acid + NADH + H+ + O2
-
 Escherichia coli cinnamic acid-2,3-dihydrodiol + NAD+
-
 ?
1.14.13.127 3-(3-hydroxyphenyl)propanoate + NADH + H+ + O2
-
 Escherichia coli 3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O
-
 ?
3.7.1.14 (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O the enzyme is involved in 3-phenylpropanoate catabolism Escherichia coli (2E)-2-hydroxypenta-2,4-dienoate + succinate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.19 tetramer gene products from hcaA1, hcaA2, hcaC and hcaD, 1 * 51109 + 1 * 20579 + 1 * 11328 + 1 * 43978, subunit masses calculated from deduced amino acid sequences Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.3.1.87 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase
-
 Escherichia coli
1.3.1.87 3-phenylpropionate-2',3'-dihydrodiol dehydrogenase
-
 Escherichia coli
1.3.1.87 3-phenylpropionate-dihydrodiol dehydrogenase
-
 Escherichia coli
1.3.1.87 HcaB
-
 Escherichia coli
1.3.1.87 HCAR HcaR controls expression of the neighbouring genes, hcaA1, hcaA2, hcaC, hcaB and hcaD, coding for the 3-phenylpropionate dioxygenase complex and 3-phenylpropionate-2',3'-dihydrodiol dehydrogenase Escherichia coli
1.14.12.19 3-phenylpropionate dioxygenase
-
 Escherichia coli
1.14.12.19 Hca dioxygenase
-
 Escherichia coli
1.14.12.19 HcaA1A2CD
-
 Escherichia coli
1.14.13.127 3-(3-hydroxyphenyl)propionate hydroxylase
-
 Escherichia coli
1.14.13.127 MhpA
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.19 NADH
-
 Escherichia coli
1.14.13.127 NADH
-
 Escherichia coli

Expression

EC Number Organism Comment Expression
1.3.1.87 Escherichia coli when bacteria are grown in the absence of oxygen, the expression of hcaR is decreased threefold compared to growth in aerobic conditions down
1.14.13.127 Escherichia coli growth with 3HCI induces the synthesis of enzyme MhpA up

General Information

EC Number General Information Comment Organism
3.7.1.14 physiological function the enzyme is involved in 3-phenylpropanoate catabolism Escherichia coli