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Literature summary extracted from

  • Halaouli, S.; Asther, M.; Kruus, K.; Guo, L.; Hamdi, M.; Sigoillot, J.C.; Lomascolo, A.
    Characterization of a new tyrosinase from Pycnoporus species with high potential for food technological applications (2005), J. Appl. Microbiol., 98, 332-343.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.9
-
 L-Dopa
-
 Trametes sanguinea
1.14.18.1 1
-
 L-Tyr
-
 Trametes sanguinea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 45000
-
 gel filtration Trametes sanguinea

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Trametes cinnabarina
-
 strain MUCL 30555, strain MUCL 29375, strain MUCL 38480, strain MUCL 39533, strain MUCL 38466, strain MUCL 38467, strain I-937, strain ss3
-
1.14.18.1 Trametes sanguinea
-
 strain CBS 357.63, strain CBS 614.73, strain G05, strain W006, strain W 006-2, strain W 3008, strain W28, strain G118, strain G66, strain H2180
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 Pycnoporus sanguineus strain 614.73 Trametes sanguinea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 30.2
-
 reaction with L-Tyr Trametes sanguinea
1.14.18.1 84.34
-
 reaction with L-dopa Trametes sanguinea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 L-dopa + 1/2 O2
-
 Trametes sanguinea L-dopaquinone + H2O
-
 ?
1.14.18.1 L-dopa + 1/2 O2
-
 Trametes cinnabarina L-dopaquinone + H2O
-
 ?
1.14.18.1 L-Tyr + O2 + AH2
-
 Trametes sanguinea L-dopa + H2O + A
-
 ?
1.14.18.1 L-Tyr + O2 + AH2
-
 Trametes cinnabarina L-dopa + H2O + A
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 monomer 1 * 45000, SDS-PAGE Trametes sanguinea

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 tyrosinase
-
 Trametes sanguinea
1.14.18.1 tyrosinase
-
 Trametes cinnabarina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 60 65
-
 Trametes sanguinea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.18.1 30
-
 30 min, about 10% loss of activity, no further inactivation after 120 min Trametes sanguinea
1.14.18.1 40
-
 120 min, about 20% loss of activity Trametes sanguinea
1.14.18.1 50
-
 120 min, about 50% loss of activity Trametes sanguinea
1.14.18.1 60
-
 20 min, almost complete inactivation Trametes sanguinea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.18.1 40.9
-
 L-Tyr
-
 Trametes sanguinea
1.14.18.1 84.85
-
 L-Dopa
-
 Trametes sanguinea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.18.1 Trametes sanguinea isoelectric focusing shows 4 bands having regular spacing and containing decreasing amounts of proteins. Corresponding to isoforms or isoenzymes of pI values between 4.5 and 5
-
 additional information