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Literature summary extracted from
- Relationship between charge-transfer interactions, redox potentials, and catalysis for different forms of the flavoprotein component of p-cresol methylhydroxylase (2005), J. Am. Chem. Soc., 127, 732-741.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.17.9.1 | expressed in Escherichia coli | Pseudomonas putida |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.17.9.1 | 9200 | - |
alpha, beta, 2 * 58700 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) | Pseudomonas putida |
| 1.17.9.1 | 58700 | - |
alpha, beta, 2 * 58700 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.9.1 | 4-cresol + acceptor + H2O | Pseudomonas putida | via intermediate 4-hydroxybenzylalcohol | 4-hydroxybenzaldehyde + reduced acceptor | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.9.1 | Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.17.9.1 | recombinant protein | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.9.1 | 4-cresol + acceptor + H2O | via intermediate 4-hydroxybenzylalcohol | Pseudomonas putida | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.17.9.1 | tetramer | alpha, beta, 2 * 58700 + 2 * 9200, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) | Pseudomonas putida |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.9.1 | 20 | - |
4-Cresol | 2-deoxy-FAD cofactor, pH 7, 25°C | Pseudomonas putida | |
| 1.17.9.1 | 121 | - |
4-Cresol | FAD cofactor, pH 7, 25°C | Pseudomonas putida | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.9.1 | 2'-deoxy-FAD | strong binding but significantly reduced activity | Pseudomonas putida | |
| 1.17.9.1 | FAD | - |
Pseudomonas putida | |
| 1.17.9.1 | additional information | only traces of activity with 6-Br-FAD, iso-FAD, 5-deaza-FAD, 6-amino-FAD, or 6-hydroxy-FAD as cofactor | Pseudomonas putida |
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Release 2023.1 (January 2023)
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