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Literature summary extracted from

  • Weigel, P.H.
    Functional characteristics and catalytic mechanisms of the bacterial hyaluronan synthases (2002), IUBMB Life, 54, 201-211.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.212 cardiolipin required for full activity, activates up to 10fold, 14-18 molecules of cardiolipin are bound to one molecule of enzyme Streptococcus pyogenes

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.212 expression as a soluble active protein comprising residues 1-703 Pasteurella multocida
2.4.1.212 overexpression in Escherichia coli, enzyme cannot be expressed as a soluble active protein Streptococcus pyogenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.212 cell membrane most parts of the enzyme, except the transmembrane region, are cytoplasmic including the active site Streptococcus pyogenes
-
-
2.4.1.212 additional information probably cytoplasm or periplasm Pasteurella multocida
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.212 Mg2+ required Streptococcus pyogenes
2.4.1.212 Mg2+ required by class II enzyme Pasteurella multocida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.212 48000
-
 1 * 48000, without cardiolipin, radiation inactivation analysis Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.212 additional information Streptococcus pyogenes enzyme is responsible for hyaluronan biosynthesis, the hyaluronan capsule is an important, but not the only, virulence factor, physiological role of the enzyme ?
-
 ?
2.4.1.212 additional information Pasteurella multocida enzyme is responsible for hyaluronan biosynthesis, the hyaluronan capsule is an important, but not the only, virulence factor, physiological role of the enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.212 Pasteurella multocida
-
 avian pathogen, class II enzyme
-
2.4.1.212 Streptococcus pyogenes
-
 human pathogen, single gene hasA in the has operon, class I enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.212 recombinant enzyme from Escherichia coli, native enzyme to homogeneity Streptococcus pyogenes

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.212 UDP-N-acetyl-alpha-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] catalytic mechanism Pasteurella multocida
a
2.4.1.212 UDP-N-acetyl-alpha-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] catalytic mechanism, no covalent intermediates Streptococcus pyogenes
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.212 additional information enzyme is responsible for hyaluronan biosynthesis, the hyaluronan capsule is an important, but not the only, virulence factor, physiological role of the enzyme Streptococcus pyogenes ?
-
 ?
2.4.1.212 additional information enzyme is responsible for hyaluronan biosynthesis, the hyaluronan capsule is an important, but not the only, virulence factor, physiological role of the enzyme Pasteurella multocida ?
-
 ?
2.4.1.212 additional information enzyme acts processive, the enzyme is active as a complex with cardiolipin, a bacterial membrane lipid Streptococcus pyogenes ?
-
 ?
2.4.1.212 additional information enzyme is not processive, enzyme requires other proteins for hyaluronan translocation Pasteurella multocida ?
-
 ?
2.4.1.212 UDP-D-glucosamine + UDP-D-glucuronate
-
 Streptococcus pyogenes [beta-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP
-
 ?
2.4.1.212 UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate addition of monosaccharides to the reducing end of the acceptor substrate, no binding and activity with exogenously added hyaluronan chains Streptococcus pyogenes [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP the product chain length can grow at the reducing end up to 40000 monosaccharides with a MW of over 8 million Da before it is released by the class I enzyme ?
2.4.1.212 UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate chains growth at the non-reducing end, which is terminated by lack of substrate with a non-reducing end, active with exogenously added acceptors substrates Pasteurella multocida [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.212 monomer 1 * 48000, without cardiolipin, radiation inactivation analysis Streptococcus pyogenes
2.4.1.212 More enzyme topology and organization, overview Streptococcus pyogenes
2.4.1.212 More predicted enzyme topology and organization, overview Pasteurella multocida

Synonyms

EC Number Synonyms Comment Organism
2.4.1.212 HA synthase
-
 Streptococcus pyogenes
2.4.1.212 HA synthase
-
 Pasteurella multocida
2.4.1.212 More the enzyme of Pasteurella multocida belongs to the group of class II hyaluronan synthases Pasteurella multocida
2.4.1.212 More the enzyme of Streptococcus pyogenes belongs to the group of class I hyaluronan synthases Streptococcus pyogenes