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Literature summary extracted from

  • Tanaka, N.; Hasan, Z.; Wever, R.
    Kinetic characterization of active site mutants Ser402Ala and Phe397His of vanadium chloroperoxidase from the fungus Curvularia inaequalis (2003), Inorg. Chim. Acta, 356, 288-296.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.B2 recombinant CPO and mutant enzymes S402A and F397H are overexpressed as apoenzyme in Saccharomyces cerevisiae Curvularia inaequalis

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.B2 F397H 12% of the activity of the wild-type enzyme (chlorination of monochlorodimedone), mutant enzyme shows enhancement of bromination activity under certain conditions, inactivation of the mutant enzyme by halide especially at low pH is observed during turnover Curvularia inaequalis
1.11.1.B2 S402A 1.8% of the activity of wild-type enzyme (chlorination of monochlorodimedone), mutation decreases activity, mutant enzyme still catalyzes efficiently oxidation of both Cl- and Br- Curvularia inaequalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.B2 Br- inhibition of mutant enzyme S402A between pH 3.5 and 4.5: concentrations greater than 0.2 mM inhibit, between pH 5.0 and 5.5: concentrations above 0.5 mM inhibit, between pH 6.0 and 6.3: 0.5 mM does not inhibit the mutant enzyme Curvularia inaequalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.B2 additional information
-
 additional information Km-value for Cl- is pH-dependent whereas the Km-value for Br- is hardly pH-dependent Curvularia inaequalis
1.11.1.B2 1.15
-
 Cl- pH 5.0, recombinant VCPO Curvularia inaequalis
1.11.1.B2 1.3
-
 H2O2 pH 6.3, mutant enzyme S402A Curvularia inaequalis
1.11.1.B2 2.6
-
 H2O2 pH 6.3, recombinant VCPO Curvularia inaequalis
1.11.1.B2 10.2
-
 Cl- pH 6.3, recombinant VCPO Curvularia inaequalis
1.11.1.B2 15
-
 Br- pH 5.0, recombinant VCPO Curvularia inaequalis
1.11.1.B2 15.3
-
 Br- pH 5.0, mutant enzyme S402A Curvularia inaequalis
1.11.1.B2 15.5
-
 Cl- pH 5.0, mutant enzyme S402A Curvularia inaequalis
1.11.1.B2 16.4
-
 Cl- pH 5.0, mutant enzyme F397H Curvularia inaequalis
1.11.1.B2 19.9
-
 Br- pH 6.3, mutant enzyme S402A Curvularia inaequalis
1.11.1.B2 25.8
-
 Br- pH 6.3, recombinant VCPO Curvularia inaequalis
1.11.1.B2 49.1
-
 H2O2 pH 5.0, recombinant VCPO Curvularia inaequalis
1.11.1.B2 66.7
-
 Br- pH 5.0, mutant enzyme F397H Curvularia inaequalis
1.11.1.B2 81.2
-
 H2O2 pH 5.0, mutant enzyme S402A Curvularia inaequalis
1.11.1.B2 174
-
 Cl- pH 6.3, mutant enzyme S402A Curvularia inaequalis
1.11.1.B2 177
-
 Br- pH pH 6.3, mutant enzyme F397H Curvularia inaequalis
1.11.1.B2 474
-
 Cl- pH pH 6.3, mutant enzyme F397H Curvularia inaequalis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.B2 Curvularia inaequalis P49053
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.B2 recombinant CPO and mutant enzymes S402A and F397H are overexpressed as apoenzyme in Saccharomyces cerevisiae Curvularia inaequalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.B2 monochlorodimedone + Br- + H2O2
-
 Curvularia inaequalis monobromo-monochlorodimedone + H2O
-
 ?
1.11.1.B2 monochlorodimedone + Cl- + H2O2
-
 Curvularia inaequalis dichlorodimedone + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.B2 vanadium chloroperoxidase
-
 Curvularia inaequalis
1.11.1.B2 vCPO
-
 Curvularia inaequalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.B2 1.13
-
 Cl- pH 5.0, mutant enzyme S402A Curvularia inaequalis
1.11.1.B2 1.42
-
 Cl- pH 6.3, mutant enzyme S402A Curvularia inaequalis
1.11.1.B2 5.7
-
 Cl- pH pH 6.3, mutant enzyme F397H Curvularia inaequalis
1.11.1.B2 6.5
-
 Cl- pH 5.0, mutant enzyme F397H Curvularia inaequalis
1.11.1.B2 7.3
-
 Cl- pH 6.3, recombinant VCPO Curvularia inaequalis
1.11.1.B2 8.1
-
 Br- pH 6.3, mutant enzyme S402A Curvularia inaequalis
1.11.1.B2 15.2
-
 H2O2 pH 6.3, recombinant VCPO Curvularia inaequalis
1.11.1.B2 21.3
-
 Br- pH 6.3, recombinant VCPO Curvularia inaequalis
1.11.1.B2 23
-
 Cl- pH 5.0, recombinant VCPO Curvularia inaequalis
1.11.1.B2 33.5
-
 Br- pH pH 6.3, mutant enzyme F397H Curvularia inaequalis
1.11.1.B2 63.8
-
 H2O2 pH 5.0, mutant enzyme S402A Curvularia inaequalis
1.11.1.B2 81.7
-
 Br- pH 5.0, mutant enzyme S402A Curvularia inaequalis
1.11.1.B2 84.4
-
 Br- pH 5.0, mutant enzyme F397H Curvularia inaequalis
1.11.1.B2 183
-
 H2O2 pH 5.0, recombinant VCPO Curvularia inaequalis
1.11.1.B2 200
-
 Br- pH 5.0, recombinant VCPO Curvularia inaequalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.B2 4.5
-
 recombinant CVPO and mutant enzyme S402A Curvularia inaequalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.B2 3.5 5.5 pH 3.5: about 70% of maximal activity, pH 5.5: about 45% of maximal activity Curvularia inaequalis