EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.2.1 | wild-type and mutant enzymes S52A, S52C and R262A are overexpressed in Escherichia coli | Ovis aries |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.2.1 | R262A | KM-value for L-Ser is 5.2fold higher than the wild-type value, the turnover-number is 5.25fold lower than the wild-type value, no transamination reaction with D-Ala, reaction with L-allo-Thr is 20fold decreased compared to wild-type value. L-Ser is unable to enhance the thermal stability as it does in wild-type enzyme | Ovis aries |
2.1.2.1 | S52A | KM-value for L-Ser is 1.3fold higher than the wild-type value, the turnover-number is 6fold lower than the wild-type value, transamination reaction with D-Ala is 20% of the wild-type activity, reaction with L-allo-Thr is 15% of the wild-type activity. L-Ser is unable to enhance the thermal stability as it does in wild-type enzyme | Ovis aries |
2.1.2.1 | S52C | KM-value for L-Ser is 11.2fold higher than the wild-type value, the turnover-number is 21fold lower than the wild-type value, no transamination reaction with D-Ala, no reaction with L-allo-Thr. L-Ser is unable to enhance the thermal stability as it does in wild-type enzyme | Ovis aries |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.1 | 1 | - |
L-Ser | pH 7.4, 37°C, wild-type enzyme | Ovis aries | |
2.1.2.1 | 1.3 | - |
L-Ser | pH 7.4, 37°C, mutant enzyme S52A | Ovis aries | |
2.1.2.1 | 5.2 | - |
L-Ser | pH 7.4, 37°C, mutant enzyme R262A | Ovis aries | |
2.1.2.1 | 11.2 | - |
L-Ser | pH 7.4, 37°C, mutant enzyme S52C | Ovis aries |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.1.2.1 | cytosol | - |
Ovis aries | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 53000 | - |
4 * 53000, SDS-PAGE | Ovis aries |
2.1.2.1 | 210000 | - |
gel filtration | Ovis aries |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.1 | Ovis aries | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.2.1 | liver | - |
Ovis aries | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.1 | 5,6,7,8-tetrahydrofolate + L-Ser | - |
Ovis aries | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.2.1 | tetramer | 4 * 53000, SDS-PAGE | Ovis aries |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.2.1 | SHMT | - |
Ovis aries |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 55 | - |
Tm-value for wild-type enzyme and mutant enzymes R262A, S52C and S52A without ligands or with Gly as ligand. Tm-value for mutant enzymes mutant enzymes R262A, S52C and S52A with L-Ser as ligand | Ovis aries |
2.1.2.1 | 68 | - |
Tm-value for wild-type enzyme with L-Ser as ligand | Ovis aries |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.1 | 0.2 | - |
L-Ser | pH 7.4, 37°C, mutant enzyme S52C | Ovis aries | |
2.1.2.1 | 0.7 | - |
L-Ser | pH 7.4, 37°C, mutant enzyme S52A | Ovis aries | |
2.1.2.1 | 0.8 | - |
L-Ser | pH 7.4, 37°C, mutant enzyme R262A | Ovis aries | |
2.1.2.1 | 4.2 | - |
L-Ser | pH 7.4, 37°C, wild-type enzyme | Ovis aries |