BRENDA - Enzyme Database

Low expression of lipid-linked oligosaccharide due to a functionally altered Dol-P-Man synthase reduces protein glycosylation in cAMP-dependent protein kinase deficient Chinese hamster ovary cells

Banerjee, D.K.; Aponte, E.; Dasilva, J.J.; Glycoconj. J. 21, 479-486 (2004)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.83
additional information
cAMP-dependent protein kinase is essential for upregulating the Dol-P-Man synthase activity and consequently the Glc3Man9GlcNAc-PP-Dol biosynthesis and protein N-glycosylation in cAMP-responsive cells
Cricetulus griseus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.00018
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, wild-type enzyme
Cricetulus griseus
2.4.1.83
0.00029
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate,enzyme from cAMP-dependent protein kinase-deficient mutant 10215
Cricetulus griseus
2.4.1.83
0.00032
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, wild-type enzyme
Cricetulus griseus
2.4.1.83
0.00045
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate,enzyme from cAMP-dependent protein kinase-deficient mutant 10215
Cricetulus griseus
2.4.1.83
0.00048
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10260
Cricetulus griseus
2.4.1.83
0.00076
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10248
Cricetulus griseus
2.4.1.83
0.00082
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10260
Cricetulus griseus
2.4.1.83
0.00093
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10248
Cricetulus griseus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.83
GDP-mannose + dolichyl phosphate
Cricetulus griseus
cAMP-dependent protein kinase is essential for upregulating the Dol-P-Man synthase activity and consequently the Glc3Man9GlcNAc-PP-Dol biosymthesis and protein N-glycosylation in cAMP-responsive cells
GDP + dolichyl D-mannosyl phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Cricetulus griseus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.83
CHO cell
-
Cricetulus griseus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDP-mannose + dolichyl phosphate
-
658867
Cricetulus griseus
GDP + dolichyl D-mannosyl phosphate
-
-
-
?
2.4.1.83
GDP-mannose + dolichyl phosphate
cAMP-dependent protein kinase is essential for upregulating the Dol-P-Man synthase activity and consequently the Glc3Man9GlcNAc-PP-Dol biosymthesis and protein N-glycosylation in cAMP-responsive cells
658867
Cricetulus griseus
GDP + dolichyl D-mannosyl phosphate
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.0000023
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, wild-type enzyme
Cricetulus griseus
2.4.1.83
0.0000029
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, wild-type enzyme
Cricetulus griseus
2.4.1.83
0.0000049
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, with or without dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10215
Cricetulus griseus
2.4.1.83
0.0000063
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10260
Cricetulus griseus
2.4.1.83
0.0000069
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10260
Cricetulus griseus
2.4.1.83
0.0000089
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10248
Cricetulus griseus
2.4.1.83
0.0000095
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10248
Cricetulus griseus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.83
additional information
cAMP-dependent protein kinase is essential for upregulating the Dol-P-Man synthase activity and consequently the Glc3Man9GlcNAc-PP-Dol biosynthesis and protein N-glycosylation in cAMP-responsive cells
Cricetulus griseus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.00018
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, wild-type enzyme
Cricetulus griseus
2.4.1.83
0.00029
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate,enzyme from cAMP-dependent protein kinase-deficient mutant 10215
Cricetulus griseus
2.4.1.83
0.00032
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, wild-type enzyme
Cricetulus griseus
2.4.1.83
0.00045
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate,enzyme from cAMP-dependent protein kinase-deficient mutant 10215
Cricetulus griseus
2.4.1.83
0.00048
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10260
Cricetulus griseus
2.4.1.83
0.00076
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10248
Cricetulus griseus
2.4.1.83
0.00082
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10260
Cricetulus griseus
2.4.1.83
0.00093
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10248
Cricetulus griseus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.83
GDP-mannose + dolichyl phosphate
Cricetulus griseus
cAMP-dependent protein kinase is essential for upregulating the Dol-P-Man synthase activity and consequently the Glc3Man9GlcNAc-PP-Dol biosymthesis and protein N-glycosylation in cAMP-responsive cells
GDP + dolichyl D-mannosyl phosphate
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.83
CHO cell
-
Cricetulus griseus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDP-mannose + dolichyl phosphate
-
658867
Cricetulus griseus
GDP + dolichyl D-mannosyl phosphate
-
-
-
?
2.4.1.83
GDP-mannose + dolichyl phosphate
cAMP-dependent protein kinase is essential for upregulating the Dol-P-Man synthase activity and consequently the Glc3Man9GlcNAc-PP-Dol biosymthesis and protein N-glycosylation in cAMP-responsive cells
658867
Cricetulus griseus
GDP + dolichyl D-mannosyl phosphate
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.0000023
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, wild-type enzyme
Cricetulus griseus
2.4.1.83
0.0000029
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, wild-type enzyme
Cricetulus griseus
2.4.1.83
0.0000049
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, with or without dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10215
Cricetulus griseus
2.4.1.83
0.0000063
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10260
Cricetulus griseus
2.4.1.83
0.0000069
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10260
Cricetulus griseus
2.4.1.83
0.0000089
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10248
Cricetulus griseus
2.4.1.83
0.0000095
-
GDP-mannose
37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10248
Cricetulus griseus