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Literature summary extracted from

  • Banerjee, D.K.; Aponte, E.; Dasilva, J.J.
    Low expression of lipid-linked oligosaccharide due to a functionally altered Dol-P-Man synthase reduces protein glycosylation in cAMP-dependent protein kinase deficient Chinese hamster ovary cells (2004), Glycoconj. J., 21, 479-486.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.83 additional information cAMP-dependent protein kinase is essential for upregulating the Dol-P-Man synthase activity and consequently the Glc3Man9GlcNAc-PP-Dol biosynthesis and protein N-glycosylation in cAMP-responsive cells Cricetulus griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.83 0.00018
-
 GDP-mannose 37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, wild-type enzyme Cricetulus griseus
2.4.1.83 0.00029
-
 GDP-mannose 37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate,enzyme from cAMP-dependent protein kinase-deficient mutant 10215 Cricetulus griseus
2.4.1.83 0.00032
-
 GDP-mannose 37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, wild-type enzyme Cricetulus griseus
2.4.1.83 0.00045
-
 GDP-mannose 37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate,enzyme from cAMP-dependent protein kinase-deficient mutant 10215 Cricetulus griseus
2.4.1.83 0.00048
-
 GDP-mannose 37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10260 Cricetulus griseus
2.4.1.83 0.00076
-
 GDP-mannose 37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10248 Cricetulus griseus
2.4.1.83 0.00082
-
 GDP-mannose 37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10260 Cricetulus griseus
2.4.1.83 0.00093
-
 GDP-mannose 37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10248 Cricetulus griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.83 GDP-mannose + dolichyl phosphate Cricetulus griseus cAMP-dependent protein kinase is essential for upregulating the Dol-P-Man synthase activity and consequently the Glc3Man9GlcNAc-PP-Dol biosymthesis and protein N-glycosylation in cAMP-responsive cells GDP + dolichyl D-mannosyl phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.83 Cricetulus griseus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.83 CHO cell
-
 Cricetulus griseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.83 GDP-mannose + dolichyl phosphate
-
 Cricetulus griseus GDP + dolichyl D-mannosyl phosphate
-
 ?
2.4.1.83 GDP-mannose + dolichyl phosphate cAMP-dependent protein kinase is essential for upregulating the Dol-P-Man synthase activity and consequently the Glc3Man9GlcNAc-PP-Dol biosymthesis and protein N-glycosylation in cAMP-responsive cells Cricetulus griseus GDP + dolichyl D-mannosyl phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.83 Dol-P-Man synthase
-
 Cricetulus griseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.83 0.0000023
-
 GDP-mannose 37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, wild-type enzyme Cricetulus griseus
2.4.1.83 0.0000029
-
 GDP-mannose 37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, wild-type enzyme Cricetulus griseus
2.4.1.83 0.0000049
-
 GDP-mannose 37°C, pH 7.0, wild-type enzyme, with or without dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10215 Cricetulus griseus
2.4.1.83 0.0000063
-
 GDP-mannose 37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10260 Cricetulus griseus
2.4.1.83 0.0000069
-
 GDP-mannose 37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10260 Cricetulus griseus
2.4.1.83 0.0000089
-
 GDP-mannose 37°C, pH 7.0, wild-type enzyme, without dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10248 Cricetulus griseus
2.4.1.83 0.0000095
-
 GDP-mannose 37°C, pH 7.0, wild-type enzyme, in presence of dolichyl phosphate, enzyme from cAMP-dependent protein kinase-deficient mutant 10248 Cricetulus griseus