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Literature summary extracted from

  • Karim, M.R.; Hashinaga, F.
    Isolation and characterization of limonoid glucosyltransferase from pummelo albedo tissue (2002), Food Chem., 76, 431-436.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.210 Ca2+ 10 mM, 13% inhibition Citrus maxima
2.4.1.210 citraconic anhydride 2 mM, 44% inhibition Citrus maxima
2.4.1.210 Cu2+ 5 mM, 58% inhibition Citrus maxima
2.4.1.210 diethyl dicarbonate 1 mM, 76% inhibition Citrus maxima
2.4.1.210 EDTA 1 mM, complete inactivation Citrus maxima
2.4.1.210 Hg2+ 1 mM, 33% inhibition Citrus maxima
2.4.1.210 Mg2+ 10 mM, 22% inhibition Citrus maxima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.210 0.065
-
 UDP-glucose pH 7.8, 37°C Citrus maxima
2.4.1.210 0.2
-
 limonin pH 7.8, 37°C Citrus maxima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.210 Co2+ 5 mM, 30% stimulation Citrus maxima
2.4.1.210 Mn2+ 5 mM, 33% stimulation Citrus maxima
2.4.1.210 Zn2+ 5 mM, 13% stimulation Citrus maxima

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.210 Citrus maxima
-
 Osbeck
-
2.4.1.210 Citrus maxima Osbeck
-
 Osbeck
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.210
-
 Citrus maxima

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.210 fruit albedo tissue Citrus maxima
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.210 0.013
-
-
 Citrus maxima

Storage Stability

EC Number Storage Stability Organism
2.4.1.210 4°C, pH 7.5, 10 mM Tris-HCl, enzyme retains 95% of the activity after 3 months, enzyme retains 73% of the activity after 9 months Citrus maxima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.210 UDP-glucose + limonin
-
 Citrus maxima glucosyl-limonin + UDP
-
 ?
2.4.1.210 UDP-glucose + limonin
-
 Citrus maxima Osbeck glucosyl-limonin + UDP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.210 37
-
-
 Citrus maxima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.210 20 60 20°C: about 40% of maximal activity, 60°C: about 40% of maximal activity Citrus maxima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.210 7.8
-
-
 Citrus maxima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.210 6.5 9.5 pH 6.5: about 70% of maximal activity, pH 9.5: about 55% of maximal activity Citrus maxima

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.210 3.5 9.5 4°C, 12 h, stable Citrus maxima
2.4.1.210 10.5
-
 4°C, 12 h, 62% loss of activity Citrus maxima