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Literature summary extracted from

  • Shigematsu, T.; Yumihara, K.; Ueda, Y.; Morimura, S.; Kida, K.
    Purification and gene cloning of the oxygenase component of the terephthalate 1,2-dioxygenase system from Delftia tsuruhatensis strain T7 (2003), FEMS Microbiol. Lett., 220, 255-260.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.15 Fe2+ bound to enzyme Delftia tsuruhatensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.15 17000
-
 alpha-beta, 3 * 46000 + 3 * 17000, SDS-PAGE Delftia tsuruhatensis
1.14.12.15 46000
-
 alpha-beta, 3 * 46000 + 3 * 17000, SDS-PAGE Delftia tsuruhatensis
1.14.12.15 235000
-
 gel filtration Delftia tsuruhatensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.15 terephthalate + NADH + O2 + H+ Delftia tsuruhatensis
-
 (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
-
 ?
1.14.12.15 terephthalate + NADH + O2 + H+ Delftia tsuruhatensis T7
-
 (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.15 Delftia tsuruhatensis Q8GRC1
-
-
1.14.12.15 Delftia tsuruhatensis T7 Q8GRC1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.15
-
 Delftia tsuruhatensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.15 2.8
-
 purified enzyme Delftia tsuruhatensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.15 terephthalate + NADH + O2 + H+
-
 Delftia tsuruhatensis (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
-
 ?
1.14.12.15 terephthalate + NADH + O2 + H+
-
 Delftia tsuruhatensis T7 (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.15 hexamer alpha-beta, 3 * 46000 + 3 * 17000, SDS-PAGE Delftia tsuruhatensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.15 iron-sulfur centre [2Fe-2S] Rieske center Delftia tsuruhatensis
1.14.12.15 NADH
-
 Delftia tsuruhatensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.12.15 Delftia tsuruhatensis isoelectric focusing, beta subunit
-
 4.9
1.14.12.15 Delftia tsuruhatensis isoelectric focusing, alpha subunit
-
 5.3