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Literature summary extracted from

  • Priestman, M.A.; Funke, T.; Singh, I.M.; Crupper, S.S.; Schonbrunn, E.
    5-Enolpyruvylshikimate-3-phosphate synthase from Staphylococcus aureus is insensitive to glyphosate (2005), FEBS Lett., 579, 728-732.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.19 overexpression in Escherichia coli strain BL21(DE3) Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 glyphosate slight inhibition, insensitivity is independent from cations, mechanism Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 additional information
-
 additional information
-
 Staphylococcus aureus
2.5.1.19 0.14
-
 3-phosphoshikimate pH 7.0, recombinant enzyme, in presence of 100 mM KCl Staphylococcus aureus
2.5.1.19 0.18
-
 phosphoenolpyruvate pH 7.0, recombinant enzyme, in presence of 100 mM KCl Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.19 Ca2+ slightly activating Staphylococcus aureus
2.5.1.19 Cs+ slightly activating Staphylococcus aureus
2.5.1.19 K+ highly activating, best at 100 mM Staphylococcus aureus
2.5.1.19 additional information no activation by Li+ and Mg2+ Staphylococcus aureus
2.5.1.19 Na+ activates Staphylococcus aureus
2.5.1.19 NH4+ highly activating Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Staphylococcus aureus penultimate step in the shikimate pathway phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Staphylococcus aureus
-
 gene aroA, class II enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.19 recombinant enzyme from Escherichia coli strain BL21(DE3) Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Staphylococcus aureus phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate penultimate step in the shikimate pathway Staphylococcus aureus phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.19 5-enolpyruvylshikimate-3-phosphate synthase
-
 Staphylococcus aureus
2.5.1.19 EPSPS
-
 Staphylococcus aureus
2.5.1.19 More class II 5-enolpyruvylshikimate-3-phosphate synthases are less sensitive to glyphosate in contrary to class I 5-enolpyruvylshikimate-3-phosphate synthases which are highly sensitive to inhibition with glyphosate Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.19 4.2
-
 3-phosphoshikimate pH 7.0, recombinant enzyme, in presence of 100 mM KCl Staphylococcus aureus
2.5.1.19 4.3
-
 phosphoenolpyruvate pH 7.0, recombinant enzyme, in presence of 100 mM KCl Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.19 7
-
 assay at Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 additional information
-
 additional information
-
 Staphylococcus aureus