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Literature summary extracted from

  • Wellmann, F.; Matern, U.; Lukacin, R.
    Significance of C-terminal sequence elements for Petunia flavanone 3beta-hydroxylase activity (2004), FEBS Lett., 561, 149-154.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.9 expression in Escherichia coli Petunia x hybrida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.9 additional information four C-terminally truncated enzyme forms are generated by deletion of five, 11, 24 or 29 amino acids. The recombinant enzymes preserve their substrate electrivity, but the specific activity decreases gradually with the extent of truncation. An enzyme chimera is constructed by domain swapping replacing the C-terminal 52 amino acids of the Petunia enzyme by equivalent region of flavonol synthase from Citrus unshiu Petunia x hybrida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.9 (2S)-flavanone + 2-oxoglutarate + O2 Petunia x hybrida key step towards biosynthesis of flavonols, anthocyanins and catechins (2R/3R)-dihydroflavonol + succinate + CO2
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Organism

EC Number Organism UniProt Comment Textmining
1.14.11.9 Petunia x hybrida
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.9 wild-type and mutant enzymes Petunia x hybrida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.9 (2S)-flavanone + 2-oxoglutarate + O2 key step towards biosynthesis of flavonols, anthocyanins and catechins Petunia x hybrida (2R/3R)-dihydroflavonol + succinate + CO2
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1.14.11.9 eriodictyol + 2-oxoglutarate + O2 95% of the activity with naringenin Petunia x hybrida (2R,3R)-dihydroquercetin + succinate + CO2
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1.14.11.9 naringenin + 2-oxoglutarate + O2
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Petunia x hybrida (2R,3R)-dihydrokaempferol + succinate + CO2
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1.14.11.9 pinocembrin + 2-oxoglutarate + O2 38% of the activity with naringenin Petunia x hybrida ?
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Synonyms

EC Number Synonyms Comment Organism
1.14.11.9 FHT
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Petunia x hybrida
1.14.11.9 flavanone 3beta-hydroxylase
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Petunia x hybrida