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A system for the heterologous expression of complex redox proteins in Rhodobacter capsulatus: characterisation of recombinant sulphite:cytochrome c oxidoreductase from Starkeya novella

Kappler, U.; McEwan, A.G.; FEBS Lett. 529, 208-214 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.8.2.1
heterologous expression in Rhodobacter capsulatus with the expression vector pDorEX, potentially useful for heterologous expression of multi-subunit proteins containing complex redox cofactors
Starkeya novella
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.2.1
0.0023
-
cytochrome c
recombinant enzyme
Starkeya novella
1.8.2.1
0.032
-
sulfite
recombinant enzyme
Starkeya novella
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.2.1
periplasm
the recombinant enzyme is secreted into the periplasm of Rhodobacter capsulatus
Starkeya novella
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.2.1
Molybdenum
0.63 per holoenzyme
Starkeya novella
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.2.1
42000
-
recombinant enzyme, analytical ultracentrifugation
Starkeya novella
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.2.1
Starkeya novella
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.2.1
-
Starkeya novella
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.8.2.1
additional information
-
-
Starkeya novella
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.2.1
sulfite + ferricyanide + H2O
-
658705
Starkeya novella
sulfate + ferrocyanide
-
-
-
?
1.8.2.1
sulfite + ferricytochrome c + H2O
-
658705
Starkeya novella
sulfate + ferrocytochrome c + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.8.2.1
dimer
alphabeta
Starkeya novella
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8.2.1
10880
-
cytochrome c
recombinant enzyme
Starkeya novella
1.8.2.1
10880
-
sulfite
recombinant enzyme
Starkeya novella
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.2.1
heme
0.91 heme c per holoenzyme
Starkeya novella
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.2.1
heterologous expression in Rhodobacter capsulatus with the expression vector pDorEX, potentially useful for heterologous expression of multi-subunit proteins containing complex redox cofactors
Starkeya novella
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.2.1
heme
0.91 heme c per holoenzyme
Starkeya novella
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.2.1
0.0023
-
cytochrome c
recombinant enzyme
Starkeya novella
1.8.2.1
0.032
-
sulfite
recombinant enzyme
Starkeya novella
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.2.1
periplasm
the recombinant enzyme is secreted into the periplasm of Rhodobacter capsulatus
Starkeya novella
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.2.1
Molybdenum
0.63 per holoenzyme
Starkeya novella
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.2.1
42000
-
recombinant enzyme, analytical ultracentrifugation
Starkeya novella
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.2.1
-
Starkeya novella
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.8.2.1
additional information
-
-
Starkeya novella
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.2.1
sulfite + ferricyanide + H2O
-
658705
Starkeya novella
sulfate + ferrocyanide
-
-
-
?
1.8.2.1
sulfite + ferricytochrome c + H2O
-
658705
Starkeya novella
sulfate + ferrocytochrome c + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.8.2.1
dimer
alphabeta
Starkeya novella
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8.2.1
10880
-
cytochrome c
recombinant enzyme
Starkeya novella
1.8.2.1
10880
-
sulfite
recombinant enzyme
Starkeya novella