EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.4.12 | overexpression of truncated wild-type and mutant enzymes as His-tagged proteins in Escherichia coli, functional coexpression of CBS1 in oocytes with Drosophila melanogaster ShC/B potassium channel | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.4.12 | C105S | site-directed mutagenesis, unaltered activity compared to the wild-type enzyme | Homo sapiens |
1.8.4.12 | C169S | site-directed mutagenesis, active site mutant, completely inactive mutant | Homo sapiens |
1.8.4.12 | W110A | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.8.4.12 | selenium | selenoprotein | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.12 | protein L-methionine (R)-sulfoxide + thioredoxin | Homo sapiens | type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins | protein L-methionine + thioredoxin disulfide | - |
? | |
1.8.4.12 | protein-L-methionine (R)-sulfoxide + dithiothreitol | Homo sapiens | type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins | protein-L-methionine + dithiothreitol disulfide + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.12 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.4.12 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | the active site selenocysteine SeC169 is essential for enzyme activity | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.8.4.12 | heart | most abundant in ventricles, interventricular septum, and apex | Homo sapiens | - |
1.8.4.12 | heart ventricle | - |
Homo sapiens | - |
1.8.4.12 | interventricular septum | - |
Homo sapiens | - |
1.8.4.12 | kidney | - |
Homo sapiens | - |
1.8.4.12 | liver | - |
Homo sapiens | - |
1.8.4.12 | additional information | tissue distribution, main expression of CBS1 in muscle tissues, overview | Homo sapiens | - |
1.8.4.12 | skeletal muscle | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.12 | protein L-methionine (R)-sulfoxide + thioredoxin | type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins | Homo sapiens | protein L-methionine + thioredoxin disulfide | - |
? | |
1.8.4.12 | protein-L-methionine (R)-sulfoxide + dithiothreitol | type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins | Homo sapiens | protein-L-methionine + dithiothreitol disulfide + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.4.12 | CBS1 | - |
Homo sapiens |
1.8.4.12 | MsrB | - |
Homo sapiens |
1.8.4.12 | peptide methionine sulfoxide reductase type B | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.4.12 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.4.12 | 7.4 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.4.12 | dithiothreitol | - |
Homo sapiens | |
1.8.4.12 | thioredoxin | - |
Homo sapiens |