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Literature summary extracted from

  • Fosdike, W.L.; Smith, T.J.; Dalton, H.
    Adventitious reactions of alkene monooxygenase reveal common reaction pathways and component interactions among bacterial hydrocarbon oxygenases (2005), FEBS J., 272, 2661-2669.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.69 H2O2
-
 Rhodococcus rhodochrous

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.69 Ethyne irreversible inhibition in the presence of NADH, suicide substrate, growth inhibition when added to the culture medium Rhodococcus rhodochrous
1.14.13.69 propyne irreversible inhibition in the presence of NADH, suicide substrate, growth inhibition when added to the culture medium Rhodococcus rhodochrous

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.69 Iron binuclear non-heme iron center Rhodococcus rhodochrous

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.69 propene + NADH + O2 Rhodococcus rhodochrous enables the growth on propene as sole carbon and energy source 1,2-epoxypropane + NAD+ + H2O
-
 ?
1.14.13.69 propene + NADH + O2 Rhodococcus rhodochrous B-276 enables the growth on propene as sole carbon and energy source 1,2-epoxypropane + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.69 Rhodococcus rhodochrous
-
 formerly Nocardia corallina, high expression of enzyme when grown with propene as sole carbon and energy source
-
1.14.13.69 Rhodococcus rhodochrous B-276
-
 formerly Nocardia corallina, high expression of enzyme when grown with propene as sole carbon and energy source
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.69
-
 Rhodococcus rhodochrous

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.69 0.145
-
 purified enzyme, pH 7.5, 30°C Rhodococcus rhodochrous

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.69 propene + NADH + O2
-
 Rhodococcus rhodochrous 1,2-epoxypropane + NAD+ + H2O
-
 ?
1.14.13.69 propene + NADH + O2 enables the growth on propene as sole carbon and energy source Rhodococcus rhodochrous 1,2-epoxypropane + NAD+ + H2O
-
 ?
1.14.13.69 propene + NADH + O2
-
 Rhodococcus rhodochrous B-276 1,2-epoxypropane + NAD+ + H2O
-
 ?
1.14.13.69 propene + NADH + O2 enables the growth on propene as sole carbon and energy source Rhodococcus rhodochrous B-276 1,2-epoxypropane + NAD+ + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.69 NADH
-
 Rhodococcus rhodochrous