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Literature summary extracted from

  • Shulami, S.; Yaniv, O.; Rabkin, E.; Shoham, Y.; Baasov, T.
    Cloning, expression, and biochemical characterization of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthase from the hyperthermophilic bacterium Aquifex pyrophilus (2003), Extremophiles, 7, 471-481.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.55 1 15.5-kb segment containing the kdsA gene, expression in Escherichia coli Aquifex pyrophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.55 C11A mutant enzyme retains less than 1% of the wild-type activity and is incapable of metal binding. Activity is not stimulated by Mn2+, Co2+ and Zn2+. Cd2+ stimulates 2fold at a concentration above 1 mM Aquifex pyrophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.55 1,10-phenanthroline
-
Aquifex pyrophilus
2.5.1.55 EDTA
-
Aquifex pyrophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.55 0.07
-
D-arabinose 5-phosphate 60°C, pH 7.0, 0.48 mM Cd2+ Aquifex pyrophilus
2.5.1.55 0.29
-
phosphoenolpyruvate 60°C, pH 7.0, 0.48 mM Cd2+ Aquifex pyrophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.55 Cd2+ stimulates sild-type enzyme above 0.4 mM, stimulation of mutant enzyme C11A above 1 mM Aquifex pyrophilus
2.5.1.55 Co2+ stimulates wild-type enzyme above 0.4 mM, no stimulation of mutant enzyme C11A Aquifex pyrophilus
2.5.1.55 Iron enzyme contains 0.19 mol of iron per mol of subunit Aquifex pyrophilus
2.5.1.55 Mn2+ stimulates wild-type enzyme above 0.4 mM, no stimulation of mutant enzyme C11A Aquifex pyrophilus
2.5.1.55 Zinc enzyme contains 0.26 mol of zinc per mol of subunit Aquifex pyrophilus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.55 Aquifex pyrophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.55 recombinant enzyme Aquifex pyrophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.55 7.2
-
-
Aquifex pyrophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate
-
Aquifex pyrophilus 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.55 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase
-
Aquifex pyrophilus
2.5.1.55 Kdo8P synthase
-
Aquifex pyrophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.55 80
-
in 10-min reaction assay Aquifex pyrophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.55 60 90 60°C: about 30% of maximal activity, 70°C: about 70% of maximal activity, 90°C: about 40% of maximal activity Aquifex pyrophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.55 70
-
half-life: 8.0 h Aquifex pyrophilus
2.5.1.55 80
-
half-life: 2.25 h Aquifex pyrophilus
2.5.1.55 90
-
half-life: 0.5 h Aquifex pyrophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.55 4
-
phosphoenolpyruvate 60°C, pH 7.0, 0.48 mM Cd2+ Aquifex pyrophilus
2.5.1.55 4
-
D-arabinose 5-phosphate 60°C, pH 7.0, 0.48 mM Cd2+ Aquifex pyrophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.55 5.5 6 in 10-min reaction assay Aquifex pyrophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.55 4.5 7.5 pH 4.5: about 50% of maximal activity, pH 7.5: about 50% of maximal activity Aquifex pyrophilus