BRENDA - Enzyme Database

Hydrolysis of diadenosine polyphosphates by nucleotide pyrophosphatases/phosphodiesterases

Vollmayer, P.; Clair, T.; Goding, J.W.; Sano, K.; Servos, J.; Zimmermann, H.; Eur. J. Biochem. 270, 2971-2978 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.6.1.9
expression in COS cells, NPP1; expression in COS cells, NPP2
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.9
Cibacron blue
0.1 mM, substrate Ap4A, strong inhibition, NPP1; 0.1 mM, substrate Ap4A, strong inhibition, NPP2
Homo sapiens
3.6.1.9
Cibacron blue
0.1 mM, substrate Ap4A, strong inhibition, NPP1
Rattus norvegicus
3.6.1.9
EDTA
NPP1; NPP2
Homo sapiens
3.6.1.9
EDTA
NPP3
Rattus norvegicus
3.6.1.9
heparan sulfate
NPP1
Homo sapiens
3.6.1.9
heparin
NPP1
Homo sapiens
3.6.1.9
additional information
NPP3 is not affected by 0.1 mM suramin
Rattus norvegicus
3.6.1.9
pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid
0.1 mM, substrate p4A, 42% inhibition, NPP2; 0.1 mM, substrate p4A, about 85% inhibition, NPP1
Homo sapiens
3.6.1.9
pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid
0.1 mM, substrate Ap4A, about 85% inhibition, NPP3
Rattus norvegicus
3.6.1.9
suramin
0.1 mM, substrate Ap4A, about 77% inhibition, NPP2; 0.1 mM, substrate p4A, about 70% inhibition, NPP1
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.9
0.0051
-
Ap3A
pH 8.5, NPP1
Homo sapiens
3.6.1.9
0.008
-
Ap3A
pH 9.0, NPP2
Homo sapiens
3.6.1.9
0.0495
-
Ap3A
pH 8.5, NPP3
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.9
membrane
of COS cells, NPP1
Homo sapiens
16020
-
3.6.1.9
membrane
of COS cells, NPP3
Rattus norvegicus
16020
-
3.6.1.9
soluble
NPP2
Homo sapiens
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.9
Ca2+
catalytic rate is similar in presence of Ca2+ and Mg2+, NPP1; catalytic rate is similar in presence of Ca2+ and Mg2+, NPP2
Homo sapiens
3.6.1.9
Ca2+
catalytic rate is similar in presence of Ca2+ and Mg2+, NPP3
Rattus norvegicus
3.6.1.9
Mg2+
catalytic rate is similar in presence of Ca2+ and Mg2+, NPP1; catalytic rate is similar in presence of Ca2+ and Mg2+, NPP2
Homo sapiens
3.6.1.9
Mg2+
catalytic rate is similar in presence of Ca2+ and Mg2+, NPP3
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.9
Homo sapiens
-
NPP1; NPP2
-
3.6.1.9
Rattus norvegicus
P97675
NPP3, recombinant
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.9
diadenosine 5',5''-P1,P3-triphosphate + H2O
NPP1
658622
Homo sapiens
5'-ADP + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P3-triphosphate + H2O
NPP2
658622
Homo sapiens
5'-ADP + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P3-triphosphate + H2O
NPP3
658622
Rattus norvegicus
5'-ADP + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
NPP1
658622
Homo sapiens
ATP + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
NPP2
658622
Homo sapiens
ATP + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
NPP3
658622
Rattus norvegicus
ATP + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
NPP1
658622
Homo sapiens
p4A + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
NPP2
658622
Homo sapiens
p4A + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
NPP3
658622
Rattus norvegicus
p4A + 5'-AMP
-
-
-
?
3.6.1.9
diguanosine 5',5'''-P1,P4-tetraphosphate + H2O
NPP1
658622
Homo sapiens
GTP + 5'-GMP
-
-
-
?
3.6.1.9
diguanosine 5',5'''-P1,P4-tetraphosphate + H2O
NPP2
658622
Homo sapiens
GTP + 5'-GMP
-
-
-
?
3.6.1.9
diguanosine 5',5'''-P1,P4-tetraphosphate + H2O
NPP3
658622
Rattus norvegicus
GTP + 5'-GMP
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.9
8.5
9
NPP1
Homo sapiens
3.6.1.9
8.5
9
NPP3
Rattus norvegicus
3.6.1.9
10
-
NPP2
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.6.1.9
7.4
10
pH 7.4: about 15% of maximal activity, pH 10: maximal activity, NPP2
Homo sapiens
3.6.1.9
7.4
8.5
pH 7.4: about 10% of maximal activity, pH 8.5-9.0: maximal activity, NPP1
Homo sapiens
3.6.1.9
7.4
8.5
pH 7.4: about 30% of maximal activity, pH 8.5-9.0: maximal activity, NPP3
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.9
expression in COS cells, NPP1; expression in COS cells, NPP2
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.9
Cibacron blue
0.1 mM, substrate Ap4A, strong inhibition, NPP1; 0.1 mM, substrate Ap4A, strong inhibition, NPP2
Homo sapiens
3.6.1.9
Cibacron blue
0.1 mM, substrate Ap4A, strong inhibition, NPP1
Rattus norvegicus
3.6.1.9
EDTA
NPP1; NPP2
Homo sapiens
3.6.1.9
EDTA
NPP3
Rattus norvegicus
3.6.1.9
heparan sulfate
NPP1
Homo sapiens
3.6.1.9
heparin
NPP1
Homo sapiens
3.6.1.9
additional information
NPP3 is not affected by 0.1 mM suramin
Rattus norvegicus
3.6.1.9
pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid
0.1 mM, substrate p4A, 42% inhibition, NPP2; 0.1 mM, substrate p4A, about 85% inhibition, NPP1
Homo sapiens
3.6.1.9
pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid
0.1 mM, substrate Ap4A, about 85% inhibition, NPP3
Rattus norvegicus
3.6.1.9
suramin
0.1 mM, substrate Ap4A, about 77% inhibition, NPP2; 0.1 mM, substrate p4A, about 70% inhibition, NPP1
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.9
0.0051
-
Ap3A
pH 8.5, NPP1
Homo sapiens
3.6.1.9
0.008
-
Ap3A
pH 9.0, NPP2
Homo sapiens
3.6.1.9
0.0495
-
Ap3A
pH 8.5, NPP3
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.9
membrane
of COS cells, NPP1
Homo sapiens
16020
-
3.6.1.9
membrane
of COS cells, NPP3
Rattus norvegicus
16020
-
3.6.1.9
soluble
NPP2
Homo sapiens
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.9
Ca2+
catalytic rate is similar in presence of Ca2+ and Mg2+, NPP1; catalytic rate is similar in presence of Ca2+ and Mg2+, NPP2
Homo sapiens
3.6.1.9
Ca2+
catalytic rate is similar in presence of Ca2+ and Mg2+, NPP3
Rattus norvegicus
3.6.1.9
Mg2+
catalytic rate is similar in presence of Ca2+ and Mg2+, NPP1; catalytic rate is similar in presence of Ca2+ and Mg2+, NPP2
Homo sapiens
3.6.1.9
Mg2+
catalytic rate is similar in presence of Ca2+ and Mg2+, NPP3
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.9
diadenosine 5',5''-P1,P3-triphosphate + H2O
NPP1
658622
Homo sapiens
5'-ADP + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P3-triphosphate + H2O
NPP2
658622
Homo sapiens
5'-ADP + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P3-triphosphate + H2O
NPP3
658622
Rattus norvegicus
5'-ADP + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
NPP1
658622
Homo sapiens
ATP + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
NPP2
658622
Homo sapiens
ATP + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
NPP3
658622
Rattus norvegicus
ATP + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
NPP1
658622
Homo sapiens
p4A + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
NPP2
658622
Homo sapiens
p4A + 5'-AMP
-
-
-
?
3.6.1.9
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
NPP3
658622
Rattus norvegicus
p4A + 5'-AMP
-
-
-
?
3.6.1.9
diguanosine 5',5'''-P1,P4-tetraphosphate + H2O
NPP1
658622
Homo sapiens
GTP + 5'-GMP
-
-
-
?
3.6.1.9
diguanosine 5',5'''-P1,P4-tetraphosphate + H2O
NPP2
658622
Homo sapiens
GTP + 5'-GMP
-
-
-
?
3.6.1.9
diguanosine 5',5'''-P1,P4-tetraphosphate + H2O
NPP3
658622
Rattus norvegicus
GTP + 5'-GMP
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.9
8.5
9
NPP1
Homo sapiens
3.6.1.9
8.5
9
NPP3
Rattus norvegicus
3.6.1.9
10
-
NPP2
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.6.1.9
7.4
10
pH 7.4: about 15% of maximal activity, pH 10: maximal activity, NPP2
Homo sapiens
3.6.1.9
7.4
8.5
pH 7.4: about 10% of maximal activity, pH 8.5-9.0: maximal activity, NPP1
Homo sapiens
3.6.1.9
7.4
8.5
pH 7.4: about 30% of maximal activity, pH 8.5-9.0: maximal activity, NPP3
Rattus norvegicus