Any feedback?
Literature summary extracted from
- Identification and characterization of thioredoxin and thioredoxin reductase from Aeropyrum pernix K1 (2002), Eur. J. Biochem., 269, 5423-5430.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | expression in Escherichia coli | Aeropyrum pernix |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.8.1.9 | FAD increases thermostability | Aeropyrum pernix |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.9 | 3.6 | - |
NADPH | - |
Aeropyrum pernix |  |
| 1.8.1.9 | 12.3 | - |
thioredoxin | - |
Aeropyrum pernix | |
| 1.8.1.9 | 172.4 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Aeropyrum pernix | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.9 | 75000 | - |
gel filtration | Aeropyrum pernix |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.9 | Aeropyrum pernix | - |
K1 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | - |
Aeropyrum pernix | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| 1.8.1.9 | thioredoxin disulfide + NADPH | - |
Aeropyrum pernix | thioredoxin + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | dimer | - |
Aeropyrum pernix |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | ApTR | - |
Aeropyrum pernix |
| 1.8.1.9 | thioredoxin reductase | - |
Aeropyrum pernix |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.9 | 90 | - |
above | Aeropyrum pernix |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.9 | 70 | 95 | 70°C: about 50% of maximal activity, 95°C: about 95% of maximal activity | Aeropyrum pernix |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.9 | 110 | - |
half-life: 4 min | Aeropyrum pernix |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.9 | 9 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Aeropyrum pernix | |
| 1.8.1.9 | 63.2 | - |
thioredoxin | - |
Aeropyrum pernix | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | FAD | - |
Aeropyrum pernix | |
| 1.8.1.9 | NADPH | - |
Aeropyrum pernix | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
