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Literature summary extracted from

  • Fischer, F.; Raimondi, D.; Aliverti, A.; Zanetti, G.
    Mycobacterium tuberculosis FprA, a novel bacterial NADPH-ferredoxin reductase (2002), Eur. J. Biochem., 269, 3005-3013.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 gene fprA, expression of the soluble enzyme in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 additional information
-
 additional information kinetics Mycobacterium tuberculosis
1.18.1.2 0.00045
-
 NADPH recombinant enzyme, pH 8.2, 25°C, with Fe(CN)63- Mycobacterium tuberculosis
1.18.1.2 0.00089
-
 NADPH recombinant enzyme, pH 8.2, 25°C, with 2,6-dichlorophenolindophenol Mycobacterium tuberculosis
1.18.1.2 0.015
-
 Fe(CN)63- recombinant enzyme, pH 8.2, 25°C, with NADH Mycobacterium tuberculosis
1.18.1.2 0.022
-
 Fe(CN)63- recombinant enzyme, pH 8.2, 25°C, with NADPH Mycobacterium tuberculosis
1.18.1.2 0.056
-
 reduced 2,6-dichlorophenolindophenol recombinant enzyme, pH 8.2, 25°C, with NADH Mycobacterium tuberculosis
1.18.1.2 0.058
-
 reduced 2,6-dichlorophenolindophenol recombinant enzyme, pH 8.2, 25°C, with NADPH Mycobacterium tuberculosis
1.18.1.2 0.068
-
 oxidized 2,6-dichlorophenolindophenol recombinant enzyme, pH 8.2, 25°C, with Fe(CN)63- Mycobacterium tuberculosis
1.18.1.2 0.083
-
 oxidized 2,6-dichlorophenolindophenol recombinant enzyme, pH 8.2, 25°C, with 2,6-dichlorophenolindophenol Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 50000
-
 1 * 50000, recombinant enzyme, SDS-PAGE Mycobacterium tuberculosis
1.18.1.2 53000
-
 recombinant enzyme, gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Mycobacterium tuberculosis
-
 gene fprA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2 soluble recombinant enzyme from Escherichia coli strain BL21(DE3) to homogeneity by ammonium sulfate fractionation, gel filtration, and anion-exchange chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.1.2 additional information
-
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 2 ferricyanide + NAD(P)H high diaphorase activity Mycobacterium tuberculosis 2 ferrocyanide + NAD(P)+ + H+
-
 ?
1.18.1.2 2,6-dichlorophenolindophenol + NADPH + H+
-
 Mycobacterium tuberculosis reduced 2,6-dichlorophenolindophenol + NADP+
-
 r
1.18.1.2 NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol high diaphorase activity Mycobacterium tuberculosis NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.18.1.2 oxidized 2,6-dichlorophenolindophenol + NADH + H+
-
 Mycobacterium tuberculosis reduced 2,6-dichlorophenolindophenol + NAD+
-
 r
1.18.1.2 reduced 2,6-dichlorophenolindophenol + NADP+
-
 Mycobacterium tuberculosis oxidized 2,6-dichlorophenolindophenol + NADPH + H+
-
 r
1.18.1.2 reduced ferredoxin + NADP+ low activity Mycobacterium tuberculosis oxidized ferredoxin + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.1.2 monomer 1 * 50000, recombinant enzyme, SDS-PAGE Mycobacterium tuberculosis
1.18.1.2 More enzyme forms aggregates in solution which can be prevented by addition of 10% glycerol and 2-mercaptoethanol Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 FprA
-
 Mycobacterium tuberculosis
1.18.1.2 NADPH ferredoxin reductase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.18.1.2 25
-
 assay at Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 21
-
 2,6-dichlorophenolindophenol recombinant enzyme, pH 8.2, 25°C, with NADH Mycobacterium tuberculosis
1.18.1.2 25.6
-
 2,6-dichlorophenolindophenol recombinant enzyme, pH 8.2, 25°C, with NADPH Mycobacterium tuberculosis
1.18.1.2 42
-
 Fe(CN)63- recombinant enzyme, pH 8.2, 25°C, with NADH Mycobacterium tuberculosis
1.18.1.2 63
-
 Fe(CN)63- recombinant enzyme, pH 8.2, 25°C, with NADPH Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.18.1.2 7.5
-
 assay at, ferric reductase activity Mycobacterium tuberculosis
1.18.1.2 8.2
-
 assay at, diaphorase activity Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 FAD 0.98 mol FAD tightly bound to 1 mol of enzyme Mycobacterium tuberculosis
1.18.1.2 NAD+ diaphorase activity, low activity with Mycobacterium tuberculosis
1.18.1.2 NADH diaphorase activity, low activity with Mycobacterium tuberculosis
1.18.1.2 NADP+
-
 Mycobacterium tuberculosis
1.18.1.2 NADPH
-
 Mycobacterium tuberculosis