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Literature summary extracted from
- Purification and characterization of active recombinant human napsin A (2000), Eur. J. Biochem., 267, 2573-2580.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.23.B1 | expression in HEK-293 cells | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.B1 | pepstatin | - |
Homo sapiens |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.B1 | 0.0017 | - |
K(dabsyl)-TSLLMAAPQ-Lucifer yellow | pH 4.7, 25°C | Homo sapiens | |
| 3.4.23.B1 | 0.0062 | - |
K(dabsyl)-TSVLMAAPQ-Lucifer yellow | pH 4.7, 25°C | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.B1 | 37000 | - |
x * 37000, deglycosylated protein, SDS-PAGE | Homo sapiens |
| 3.4.23.B1 | 39000 | - |
x * 39000, glycosylated protein, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.B1 | K(dabsyl)-TSLLMAAPQ-Lucifer yellow + H2O | Homo sapiens | - |
K(dabsyl)-TSLL + MAAPQ-Lucifer yellow | - |
? | |
| 3.4.23.B1 | polypeptide + H2O | Homo sapiens | - |
peptides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.B1 | Homo sapiens | O96009 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.B1 | recombinant from HEK-293 cells, to homogeneity, 1-step procedure with propeptide of napsin A as affinity ligand | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.B1 | K(dabsyl)-TSLLMAAPQ-Lucifer yellow + H2O | - |
Homo sapiens | K(dabsyl)-TSLL + MAAPQ-Lucifer yellow | - |
? | |
| 3.4.23.B1 | K(dabsyl)-TSVLMAAPQ-Lucifer yellow + H2O | - |
Homo sapiens | K(dabsyl)-TSVL + MAAPQ-Lucifer yellow | - |
? | |
| 3.4.23.B1 | polypeptide + H2O | - |
Homo sapiens | peptides | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.23.B1 | ? | x * 37000, deglycosylated protein, SDS-PAGE | Homo sapiens |
| 3.4.23.B1 | ? | x * 39000, glycosylated protein, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.B1 | 25 | - |
- |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.B1 | 4 | 5.5 | recombinant enzyme, at room temperature | Homo sapiens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.B1 | 5 | - |
10 h, 37°C, stable | Homo sapiens |
| 3.4.23.B1 | 6 | - |
10 h, 37°C, stable | Homo sapiens |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.B1 | 0.000002 | - |
pepstatin | pH 4.7, 25°C | Homo sapiens | |
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