EC Number | General Stability | Organism |
---|---|---|
3.4.21.B3 | methanol 1% v/v stabilizes the purified enzyme | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.B3 | alpha-N-tosyl-L-lysine chloromethane | - |
Bos taurus | |
3.4.21.B3 | Bowman Birk soybean inhibitor | - |
Bos taurus | |
3.4.21.B3 | chymostatin | - |
Bos taurus | |
3.4.21.B3 | chymotrypsin inhibitor | form I and II from potato, from kidney bean | Bos taurus | |
3.4.21.B3 | diisopropylphosphofluoridate | complete inhibition, irreversible inhibition | Bos taurus | |
3.4.21.B3 | EDTA | weak inhibition at high concentration | Bos taurus | |
3.4.21.B3 | o-phenanthroline | weak | Bos taurus | |
3.4.21.B3 | phenylmethanesulfonyl fluoride | irreversible inhibition | Bos taurus | |
3.4.21.B3 | Trypsin inhibitor | from tree-thorned acacia, from kidney bean, from soybean, from Lima beans | Bos taurus | |
3.4.21.B3 | white ovomucoid | from chicken egg | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.B3 | 1.1 | - |
alpha-N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
3.4.21.B3 | 1.1 | - |
alpha-N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
3.4.21.B3 | 1.2 | - |
alpha-N-succinyl-Val-Pro-Phe-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
3.4.21.B3 | 1.3 | - |
alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
3.4.21.B3 | 1.5 | - |
alpha-N-succinyl-Gly-Gly-Phe-4-nitroanilide | pH 8.0, 37°C | Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.21.B3 | 24970 | - |
mass spectrometry | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.B3 | polypeptide + H2O | Bos taurus | - |
peptides | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.B3 | Bos taurus | P80219 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.B3 | at least 90% purity | Bos taurus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.4.21.B3 | proteolytic cleavage of polypeptides to large and stable peptides, preferential cleavage sites: Lys-, Arg-, Tyr-, Phe-, Leu-, -Pro | active site serine | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.21.B3 | duodenum | - |
Bos taurus | - |
3.4.21.B3 | mucosa | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.21.B3 | 0.162 | - |
purified enzyme | Bos taurus |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.21.B3 | -20°C, purified enzyme, in water stable for at least 1 year, lyophilized stable for several months | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.B3 | alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-succinyl-Ala-Ala-Phe + 4-nitroaniline | - |
? | |
3.4.21.B3 | alpha-N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline | - |
? | |
3.4.21.B3 | alpha-N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? | |
3.4.21.B3 | alpha-N-succinyl-Gly-Gly-Phe-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-succinyl-Gly-Gly-Phe + 4-nitroaniline | - |
? | |
3.4.21.B3 | alpha-N-succinyl-Val-Pro-Phe-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-succinyl-Val-Pro-Phe + 4-nitroaniline | - |
? | |
3.4.21.B3 | polypeptide + H2O | - |
Bos taurus | peptides | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.21.B3 | monomer | 1 * 23000-24000, nonreducing SDS-PAGE, 1 * 27000-28000, reducing SDS-PAGE | Bos taurus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.B3 | ChlD | - |
Bos taurus |
3.4.21.B3 | chymotrypsin-like duodenase | - |
Bos taurus |
3.4.21.B3 | duodenase I | - |
Bos taurus |
3.4.21.B3 | duodenase serine protease | - |
Bos taurus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.B3 | 37 | - |
10 min, in solution at neutral pH, loss of 30% activity | Bos taurus |
3.4.21.B3 | 70 | - |
stable up to | Bos taurus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.B3 | 1.2 | - |
alpha-N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
3.4.21.B3 | 3.6 | - |
alpha-N-succinyl-Gly-Gly-Phe-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
3.4.21.B3 | 13.8 | - |
alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
3.4.21.B3 | 68.4 | - |
alpha-N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
3.4.21.B3 | 168 | - |
alpha-N-succinyl-Val-Pro-Phe-4-nitroanilide | pH 8.0, 37°C | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.B3 | 10 | - |
broad optimum at pH 8.0-10.5, maximum at pH 10.0 | Bos taurus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.B3 | 3 | 10 | stable | Bos taurus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.4.21.B3 | Bos taurus | above | - |
10 |