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Literature summary extracted from

  • Nachiyar, C.V.; Rajakumar, G.S.
    Purification and characterization of an oxygen insensitive azoreductase from Pseudomonas aeruginosa (2005), Enzyme Microb. Technol., 36, 503-509.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.7.1.6 environmental protection potential for the treatment of azo dye contaminated wastewater Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.6 Cu2+
-
 Pseudomonas aeruginosa
1.7.1.6 Fe2+ complete inhibition Pseudomonas aeruginosa
1.7.1.6 Hg2+
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.6 0.063
-
 Navitan fast blue S5R pH 7.0, room temperature Pseudomonas aeruginosa
1.7.1.6 4.35
-
 NADH pH 7.0, room temperature Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.6 29000
-
 SDS-PAGE, native PAGE, gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.6
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 Amaranth + NADH
-
 Pseudomonas aeruginosa ?
-
 ?
1.7.1.6 Amido black + NADH
-
 Pseudomonas aeruginosa ?
-
 ?
1.7.1.6 Atul acid black + NADH
-
 Pseudomonas aeruginosa ?
-
 ?
1.7.1.6 Eriochrome blue black + NADH
-
 Pseudomonas aeruginosa ?
-
 ?
1.7.1.6 Navitan fast blue S5R + NADH
-
 Pseudomonas aeruginosa metanilic acid + 1,4-diamino naphtalene + peri acid + NAD+
-
 ?
1.7.1.6 Orange G + NADH
-
 Pseudomonas aeruginosa ?
-
 ?
1.7.1.6 Tropaeolin + NADH
-
 Pseudomonas aeruginosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.6 monomer 1 * 29000, subunit mass by SDS-PAGE, native mass by gel filtration Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.6 35
-
-
 Pseudomonas aeruginosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.7.1.6 25 50
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.6 7
-
-
 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.1.6 5 9
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 NADH
-
 Pseudomonas aeruginosa