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Literature summary extracted from

  • Kuiper, G.G.; Klootwijk, W.; Visser, T.J.
    Substitution of cysteine for a conserved alanine residue in the catalytic center of type II iodothyronine deiodinase alters interaction with reducing cofactor (2002), Endocrinology, 143, 1190-1198.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.99.4 expression in COS cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.21.99.4 A131C similar Km-values for L-thyroxine and 3,3',5-triiodothyronine as the wild-type enzyme. Mutation improves the interaction with the reducing cofactor dithiothreitol Homo sapiens
1.21.99.4 A131S similar Km-values for L-thyroxine and 3,3',5-triiodothyronine as the wild-type enzyme. Mutation improves the interaction with the reducing cofactor dithiothreitol Homo sapiens
1.21.99.4 SeC133C mutant enzyme with a 1000fold increase in Km-value for L-thyroxine Homo sapiens
1.21.99.4 SeC133C/A131C mutant enzyme with more than 1000fold increase in Km-value for L-thyroxine Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.99.4 gold thioglucose
-
 Homo sapiens
1.21.99.4 iodoacetate
-
 Homo sapiens
1.21.99.4 propylthiouracil wild-type and mutant enzymes A131C and A131S are insensitive to propylthiouracil in presence ogf 20 mM dithiothreitol. When tested in presence of 0.2 mM dithiothreitol the Ic50 value for propylthiouracil is reduced to about 0.1 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21.99.4 0.00000101
-
 reverse triiodothyronine mutant enzyme A131S, in presence of 20 mM dithiothreitol Homo sapiens
1.21.99.4 0.0000045
-
 L-thyroxine mutant enzyme A131C, in presence of 20 mM dithiothreitol Homo sapiens
1.21.99.4 0.0000049
-
 L-thyroxine wild-type enzyme, in presence of 20 mM dithiothreitol Homo sapiens
1.21.99.4 0.0000054
-
 L-thyroxine mutant enzyme A131S, in presence of 20 mM dithiothreitol Homo sapiens
1.21.99.4 0.0000089
-
 reverse triiodothyronine wild-type enzyme, in presence of 20 mM dithiothreitol Homo sapiens
1.21.99.4 0.0000098
-
 reverse triiodothyronine mutant enzyme A131C, in presence of 20 mM dithiothreitol Homo sapiens
1.21.99.4 0.005
-
 L-thyroxine mutant enzyme SeC133C, in presence of 20 mM dithiothreitol Homo sapiens
1.21.99.4 0.0066
-
 L-thyroxine mutant enzyme SeC133C/A131C, in presence of 20 mM dithiothreitol Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.99.4 Se contains selenocysteine in the active center Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.21.99.4 Homo sapiens Q92813
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.99.4 3,3',5-triiodothyronine + AH2 rT3 ORD activity Homo sapiens diiodothyronine + iodide + A + H+
-
 ?
1.21.99.4 L-thyroxine + AH2 reaction with dithiothreitol Homo sapiens 3,3',5-triiodothyronine + iodide + A + H+
-
 ?
1.21.99.4 reverse triiodothyronine + AH2
-
 Homo sapiens diiodothyronine + iodide + A + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.21.99.4 type II iodothyronine deiodinase
-
 Homo sapiens