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Literature summary extracted from

  • Jinno, H.; Saeki, M.; Tanaka-Kagawa, T.; Hanioka, N.; Saito, Y.; Ozawa, S.; Ando, M.; Shirao, K.; Minami, H.; Ohtsu, A.; Yoshida, T.; Saijo, N.; Sawada, J.
    Functional characterization of wild-type and variant (T202I and M59I) human UDP-glucuronosyltransferase 1A10 (2003), Drug Metab. Dispos., 31, 528-532.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.17 DNA and amino acid sequence determination and analyis of wild-type isozyme UGT1A10, expression of wild-type and mutant isozymes UGT1A10 in COS-1 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.17 M59I mutation in isozyme UGT1A10, site-directed mutagenesis, activity similar to the wild-type enzyme Homo sapiens
2.4.1.17 T202I mutation in isozyme UGT1A10, site-directed mutagenesis, about 50% reduced activity compared to the wild-type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.17 0.00192
-
 17beta-estradiol recombinant mutant M59I, pH 7.5, 37°C Homo sapiens
2.4.1.17 0.00233
-
 17beta-estradiol recombinant mutant T202I, pH 7.5, 37°C Homo sapiens
2.4.1.17 0.00234
-
 17beta-estradiol recombinant wild-type enzyme, pH 7.5, 37°C Homo sapiens
2.4.1.17 0.00418
-
 7-hydroxy-4-trifluoromethylcoumarin recombinant mutant M59I, pH 7.5, 37°C Homo sapiens
2.4.1.17 0.00491
-
 7-hydroxy-4-trifluoromethylcoumarin recombinant wild-type enzyme, pH 7.5, 37°C Homo sapiens
2.4.1.17 0.00537
-
 7-hydroxy-4-trifluoromethylcoumarin recombinant mutant T202I, pH 7.5, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.17 membrane
-
 Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.17 Mg2+
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.17 Homo sapiens Q9HAW8 isozyme UGT1A10; isozyme UGT1A10
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.17 stomach isozyme UGT1A10 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.17 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.17 UDP-glucuronate + 17beta-estradiol
-
 Homo sapiens UDP + 17beta-estradiol 3-O-glucuronide
-
 ?
2.4.1.17 UDP-glucuronate + 7-hydroxy-4-trifluoromethylcoumarin
-
 Homo sapiens UDP + 7-hydroxy-4-trifluoromethylcoumarin beta-D-glucuronide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.17 UDP-glucuronosyltransferase 1A10
-
 Homo sapiens
2.4.1.17 UGT1A10
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.17 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.17 7.5
-
 assay at Homo sapiens