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Literature summary extracted from

  • Stone, A.N.; MacKenzie, P.I.; Galetin, A.; Houston, B.J.; Miners, J.O.
    Isoform selectivity and kinetics of morphine 3- and 6-glucuronidation by human UDP-glucuronosyltransferases: Evidence for atypical glucuronidation kinetics by UGT2B7 (2003), Drug Metab. Dispos., 31, 1086-1089.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.17 stable expression of isozymes UGT1A1, UGT1A3, UGT1A6, UGT1A8, UGT1A9, UGT1A10, and UGT2B7 in HEK 293 cells, isozymes UGT2B4, UGT2B7, UGT2B10, and UGT2B15 are inactivated during cell lysis for microsome preparation Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.17 additional information
-
 additional information kinetics Homo sapiens
2.4.1.17 0.42
-
 morphine morphine 3-glucuronide formation, recombinant isozyme UGT2B7, pH 7.4, 37°C Homo sapiens
2.4.1.17 0.97
-
 morphine morphine 6-glucuronide formation, recombinant isozyme UGT2B7, pH 7.4, 37°C Homo sapiens
2.4.1.17 2.6
-
 morphine morphine 3-glucuronide formation, recombinant isozyme UGT1A8, pH 7.4, 37°C Homo sapiens
2.4.1.17 3.2
-
 morphine morphine 3-glucuronide formation, recombinant isozyme UGT1A3, pH 7.4, 37°C Homo sapiens
2.4.1.17 12.6
-
 morphine morphine 3-glucuronide formation, recombinant isozyme UGT1A10, pH 7.4, 37°C Homo sapiens
2.4.1.17 18
-
 morphine morphine 3-glucuronide formation, recombinant isozyme UGT1A6, pH 7.4, 37°C Homo sapiens
2.4.1.17 18.7
-
 morphine morphine 3-glucuronide formation, recombinant isozyme UGT1A1, pH 7.4, 37°C Homo sapiens
2.4.1.17 37.4
-
 morphine morphine 3-glucuronide formation, recombinant isozyme UGT1A9, pH 7.4, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.17 membrane
-
 Homo sapiens 16020
-
2.4.1.17 microsome
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.17 additional information Homo sapiens isozyme UGT2B7 is the major isozyme involved in metabolization of morphine in the liver ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.17 Homo sapiens
-
 several enzyme isoforms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.17 microsome preparation of HEK 293 cells expressing recombinant isozymes Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.17 gastrointestinal tract isozymes UGT1A1, UGT1A3, UGT1A6, UGT1A8, UGT1A9, UGT1A10, and UGT2B7 Homo sapiens
-
2.4.1.17 liver isozymes UGT1A1, UGT1A3, UGT1A6, UGT1A9, and UGT2B7 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.17 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.17 additional information isozyme UGT2B7 is the major isozyme involved in metabolization of morphine in the liver Homo sapiens ?
-
 ?
2.4.1.17 UDP-glucuronate + 4-methylumbelliferone all isozymes, nonselective substrate Homo sapiens UDP + 4-methylumbelliferyl-beta-D-glucuronide
-
 ?
2.4.1.17 UDP-glucuronate + morphine isozymes UGT1A1, UGT1A3, UGT1A6, UGT1A8, UGT1A9, UGT1A10, and UGT2B7 Homo sapiens UDP + morphine-3-beta-D-glucuronide
-
 ?
2.4.1.17 UDP-glucuronate + morphine isozyme UGT2B7 Homo sapiens UDP + morphine-6-beta-D-glucuronide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.17 More enzymes belongs to the 2B subfamily Homo sapiens
2.4.1.17 UDP-glucuronosyltransferase
-
 Homo sapiens
2.4.1.17 UGT
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.17 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.17 7.4
-
 assay at Homo sapiens