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Literature summary extracted from

  • Macrides, T.A.; Faktor, D.A.; Kalafatis, N.; Amiet, R.G.
    Enzymic sulfation of bile salts. Partial purification and characterization of an enzyme from the liver of the shark Heterodontus portusjacksoni that catalyses the sulfation of the shark bile steroid 5 beta-scymnol (1994), Comp. Biochem. Physiol. B Biochem. Mol. Biol., 107, 461-469.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.32 adenosine-3',5'-diphosphate 0.1 mM, 98% inhibition Heterodontus portusjacksoni
2.8.2.32 iodoacetate 0.1 mM, 99% inhibition Heterodontus portusjacksoni
2.8.2.32 NaN3 0.1 mM, 65% inhibition Heterodontus portusjacksoni
2.8.2.32 p-chloromercuribenzoate 0.1 mM, 67% inhibition Heterodontus portusjacksoni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.32 0.004
-
 3'-phosphoadenosine-5'-phosphosulfate pH 6.5 Heterodontus portusjacksoni
2.8.2.32 0.014
-
 5beta-scymnol pH 6.5 Heterodontus portusjacksoni

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.32 cytosol
-
 Heterodontus portusjacksoni 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.32 40000
-
 gel filtration Heterodontus portusjacksoni
2.8.2.32 40000
-
 native PAGE, probably two isoenzymes Heterodontus portusjacksoni
2.8.2.32 45000
-
 native PAGE, probably two isoenzymes Heterodontus portusjacksoni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.32 3'-phosphoadenosine 5'-phosphosulfate + 5beta-scymnol Heterodontus portusjacksoni
-
 adenosine 3',5'-diphosphate + 5beta-scymnol sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.32 Heterodontus portusjacksoni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.32 hydroxylapatite, Sephadex G-100 Heterodontus portusjacksoni

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.32 liver
-
 Heterodontus portusjacksoni
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.32 0.0000000275
-
-
 Heterodontus portusjacksoni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.32 3'-phosphoadenosine 5'-phosphosulfate + 5beta-scymnol
-
 Heterodontus portusjacksoni adenosine 3',5'-diphosphate + 5beta-scymnol sulfate
-
 ?
2.8.2.32 3'-phosphoadenosine 5'-phosphosulfate + 5beta-scymnol sulfation occurs at either the C-26 or C-27 position Heterodontus portusjacksoni adenosine 3',5'-diphosphate + 5beta-scymnol sulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.32 monomer 1 * 40000, SDS-PAGE, probably two isoenzymes Heterodontus portusjacksoni
2.8.2.32 monomer 1 * 45000, SDS-PAGE, probably two isoenzymes Heterodontus portusjacksoni

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.2.32 Heterodontus portusjacksoni isoelectric focusing, probably two isoenzymes
-
 5
2.8.2.32 Heterodontus portusjacksoni isoelectric focusing, probably two isoenzymes
-
 5.85