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Literature summary extracted from

  • Pettigrew, N.E.; Wright, P.F.A.; Macrides, T.A.
    5beta-Scymnol sulfotransferases from the liver of two Australian ray species (1998), Comp. Biochem. Physiol. B, 121B, 341-348.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.32 adenosine-3',5'-diphosphate 0.1 mM, 95% inhibition Trygonoptera sp.
2.8.2.32 adenosine-3',5'-diphosphate 0.1 mM, 96% inhibition Trygonorrhina fasciata
2.8.2.32 iodoacetic acid 0.1 mM, 16% inhibition Trygonoptera sp.
2.8.2.32 iodoacetic acid 0.1 mM, 40% inhibition Trygonorrhina fasciata
2.8.2.32 NaN3 0.1 mM, 24% inhibition Trygonoptera sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.32 0.0059
-
 (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol
-
 Trygonorrhina fasciata
2.8.2.32 0.018
-
 (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5-beta-cholestane-27-ol
-
 Trygonoptera sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.32 cytosol
-
 Trygonorrhina fasciata 5829
-
2.8.2.32 cytosol
-
 Trygonoptera sp. 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.32 Mg2+ 0.5 mM, 35% activation Trygonorrhina fasciata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.32 66000
-
 gel filtration Trygonorrhina fasciata
2.8.2.32 69000
-
 gel filtration Trygonoptera sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.32 3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol Trygonorrhina fasciata trivial name 5beta-scymnol adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate trivial name 5beta-scymnol sulfate ?
2.8.2.32 3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol Trygonoptera sp. trivial name 5beta-scymnol adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate trivial name 5beta-scymnol sulfate ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.32 Trygonoptera sp.
-
-
-
2.8.2.32 Trygonorrhina fasciata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.32 hydroxylapatite, DEAE-Sephacel Trygonorrhina fasciata
2.8.2.32 Sephadex G-100, hydroxylapatite Trygonoptera sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.32 kidney
-
 Trygonorrhina fasciata
-
2.8.2.32 kidney
-
 Trygonoptera sp.
-
2.8.2.32 liver
-
 Trygonorrhina fasciata
-
2.8.2.32 liver
-
 Trygonoptera sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.32 0.0000026
-
-
 Trygonoptera sp.
2.8.2.32 0.0000035
-
-
 Trygonorrhina fasciata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.32 3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5-beta-cholestane-27-yl trivial name 5beta-scymnol Trygonorrhina fasciata adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-yl sulfate trivial name 5beta-scymnol sulfate ?
2.8.2.32 3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol trivial name 5beta-scymnol Trygonorrhina fasciata adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate trivial name 5beta-scymnol sulfate ?
2.8.2.32 3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol trivial name 5beta-scymnol Trygonoptera sp. adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate trivial name 5beta-scymnol sulfate ?
2.8.2.32 3'-phosphoadenosine 5'-phosphosulfate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-ol trivial name 5beta-scymnol Trygonoptera sp. adenosine 3',5'-diphosphate + (24R)-3alpha,7alpha,12alpha,24,26-pentahydroxy-5beta-cholestane-27-sulfate trivial name beta-scymnol sulfate ?
2.8.2.32 3'-phosphoadenosine 5'-phosphosulfate + 5alpha-cyprinol 174% of activity with 5beta-scymnol Trygonorrhina fasciata adenosine 3',5'-diphosphate + 5alpha-cyprinol sulfate
-
 ?
2.8.2.32 3'-phosphoadenosine 5'-phosphosulfate + 5alpha-cyprinol 250% of activity with 5beta-scymnol Trygonoptera sp. adenosine 3',5'-diphosphate + 5alpha-cyprinol sulfate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.32 30
-
-
 Trygonorrhina fasciata
2.8.2.32 30
-
-
 Trygonoptera sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.8.2.32 22 37
-
 Trygonoptera sp.
2.8.2.32 25 45
-
 Trygonorrhina fasciata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.32 6.5
-
-
 Trygonorrhina fasciata
2.8.2.32 6.5
-
-
 Trygonoptera sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.32 5 7
-
 Trygonorrhina fasciata
2.8.2.32 5.5 7.5
-
 Trygonoptera sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.32 0.00098
-
 adenosine-3',5'-diphosphate
-
 Trygonoptera sp.
2.8.2.32 0.019
-
 adenosine-3',5'-diphosphate
-
 Trygonorrhina fasciata