Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cadicamo, C.D.; Courtieu, J.; Deng, H.; Meddour, A.; O'Hagan, D.
    Enzymatic fluorination in Streptomyces cattleya takes place with an inversion of configuration consistent with an SN2 reaction mechanism (2004), Chembiochem, 5, 685-690.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.63 K+ in the coupled assay with SAM synthase and fluorinase Streptomyces cattleya
2.5.1.63 Mg2+ in the coupled assay with SAM synthase and fluorinase Streptomyces cattleya

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + fluoride Streptomyces cattleya
-
 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.63 Streptomyces cattleya
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.63 S-adenosyl-L-methionine + fluoride = 5'-deoxy-5'-fluoroadenosine + L-methionine reaction mechanism, inversion of configuration consistent with a SN2 reaction mechanism Streptomyces cattleya
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.63 additional information
-
 development of a coupled assay with SAM synthase and fluorinase Streptomyces cattleya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + fluoride
-
 Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?
2.5.1.63 S-adenosyl-L-methionine + fluoride reaction mechanism analysis utilizing a stereospecifically labelled substrate (5'R)-[5'-2H]-ATP in a coupled assay, inversion of configuration consistent with a SN2 reaction mechanism Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine i.e. 5'-FDA, NMR product analysis ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.63 5'-FDA synthase
-
 Streptomyces cattleya
2.5.1.63 5'-FDAS
-
 Streptomyces cattleya

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.63 37
-
 assay at Streptomyces cattleya