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Literature summary extracted from

  • Shao, J.; Zhang, J.; Kowal, P.; Lu, Y.; Wang, P.G.
    Efficient synthesis of globoside and isogloboside tetrasaccharides by using beta(1->3) N-acetylgalactosaminyltransferase/UDP-N-acetylglucosamine C4 epimerase fusion protein (2003), Chem. Commun. (Camb.), 2003, 1422-1423.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.4.1.79 synthesis synthesis of globoside and isogloboside tetrasaccharides by using beta(1->3) N-acetylgalactosaminyltransferase/UDP-N-acetylglucosamine C4 epimerase fusion protein Haemophilus influenzae

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.79 additional information preparation of a bifunctional beta(1->3) N-acetylgalactosaminyltransferase/UDP-N-acetylglucosamine C4 epimerase fusion protein by in-frame linking of the Plesiomonas shigelloides wbgU gene downstream to the Haemophilus influenzae lgtD gene through a five-residue peptide linker (Thr-Gly-Gly-Gly-Gly). The enzyme is expressed in Escherichia coli BL21 at a relatively high level Haemophilus influenzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.79 additional information
-
 MW of the bifunctional beta(1,3)-N-acetylgalactosaminyltransferase/UDP-N-acetylglucosamine C4 epimerase fusion protein is 70000 Da Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.79 Haemophilus influenzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.79 one-step purification of the bifunctional beta(1->3) N-acetylgalactosaminyltransferase/UDP-N-acetylglucosamine C4 epimerase fusion protein Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.79 UDP-N-acetyl-D-galactosamine + Galalpha1,3Galbeta1,4Glc
-
 Haemophilus influenzae UDP + GalNAcbeta1,3-D-Galalpha1,3Galbeta1,4Glc
-
 ?
2.4.1.79 UDP-N-acetyl-D-galactosamine + Galalpha1,3Galbeta1,4Glc-NHAc
-
 Haemophilus influenzae UDP + GalNAcbeta1,3-D-Galalpha1,3Galbeta1,4Glc-NHAc
-
 ?
2.4.1.79 UDP-N-acetyl-D-galactosamine + Galalpha1,3Galbeta1,4Glc-OBn
-
 Haemophilus influenzae UDP + GalNAcbeta1,3-D-Galalpha1,3Galbeta1,4Glc-OBn
-
 ?
2.4.1.79 UDP-N-acetyl-D-galactosamine + Galalpha1,3Galbeta1,4Glc-OMe
-
 Haemophilus influenzae UDP + GalNAcbeta1,3-D-Galalpha1,3Galbeta1,4Glc-OMe
-
 ?
2.4.1.79 UDP-N-acetyl-D-galactosamine + Galalpha1,3Galbeta1,4Glc-SPh
-
 Haemophilus influenzae UDP + GalNAcbeta1,3-D-Galalpha1,3Galbeta1,4Glc-SPh
-
 ?
2.4.1.79 UDP-N-acetyl-D-galactosamine + Galalpha1,3Galbeta1,4GlcNAc
-
 Haemophilus influenzae UDP + GalNAcbeta1,3-D-Galalpha1,3Galbeta1,4GlcNAc
-
 ?
2.4.1.79 UDP-N-acetyl-D-galactosamine + Galalpha1,4Galbeta1,4Glc
-
 Haemophilus influenzae UDP + GalNAcbeta1,3-D-Galalpha1,4Galbeta1,4Glc
-
 ?
2.4.1.79 UDP-N-acetyl-D-galactosamine + Galalpha1,4Galbeta1,4Glc-N3
-
 Haemophilus influenzae UDP + GalNAcbeta1,3-D-Galalpha1,4Galbeta1,4Glc-N3
-
 ?
2.4.1.79 UDP-N-acetyl-D-galactosamine + Galalpha1,4Galbeta1,4Glc-OBn
-
 Haemophilus influenzae UDP + GalNAcbeta1,3-D-Galalpha1,4Galbeta1,4Glc-OBn
-
 ?
2.4.1.79 UDP-N-acetyl-D-galactosamine + Galalpha1,4Galbeta1,4Glc-OMe
-
 Haemophilus influenzae UDP + GalNAcbeta1,3-D-Galalpha1,4Galbeta1,4Glc-OMe
-
 ?
2.4.1.79 UDP-N-acetyl-D-galactosamine + Galalpha1,4Galbeta1,4Glc-SPh
-
 Haemophilus influenzae UDP + GalNAcbeta1,3-D-Galalpha1,4Galbeta1,4Glc-SPh
-
 ?
2.4.1.79 UDP-N-acetyl-D-galactosamine + Galalpha1,4Galbeta1,4GlcNAc
-
 Haemophilus influenzae UDP + GalNAcbeta1,3-D-Galalpha1,4Galbeta1,4GlcNAc
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.79 beta(1->3) N-acetylgalactosaminyltransferase
-
 Haemophilus influenzae