Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hsu, J.H.; Yang, C.C.; Su, J.C.; Lee, P.D.
    Purification and characterization of a cytosolic starch phosphorylase from etiolated rice seedlings (2004), Bot. Bull. Acad. Sin., 45, 187-196.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 2-mercaptoethanol slight activation Oryza sativa
2.4.1.1 DTT slight activation Oryza sativa
2.4.1.1 glutathione slight activation Oryza sativa
2.4.1.1 L-cysteine slight activation Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 ADP-glucose strong inhibition Oryza sativa
2.4.1.1 AMP slight inhibition Oryza sativa
2.4.1.1 Cyclodextrins
-
 Oryza sativa
2.4.1.1 D-fructose 6-phosphate strong inhibition Oryza sativa
2.4.1.1 D-mannose 1-phosphate strong inhibition Oryza sativa
2.4.1.1 IMP slight inhibition Oryza sativa
2.4.1.1 additional information no or poor inhibition by ribose 5-phosphate, glucose 6-phosphate, fructose-1,6-bisphosphate, ADP and UDP Oryza sativa
2.4.1.1 UDP-glucose strong inhibition Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 0.0046
-
 amylopectin pH 5.5, 37°C, synthesis reaction Oryza sativa
2.4.1.1 0.0106
-
 soluble starch pH 5.5, 37°C, synthesis reaction Oryza sativa
2.4.1.1 0.0138
-
 glycogen pH 5.5, 37°C, synthesis reaction Oryza sativa
2.4.1.1 0.0261
-
 soluble starch pH 5.5, 37°C, synthesis reaction Oryza sativa
2.4.1.1 0.0418
-
 Dextrin pH 5.5, 37°C, synthesis reaction Oryza sativa
2.4.1.1 2.1
-
 alpha-D-glucose 1-phosphate pH 5.5, 37°C, synthesis reaction Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.1 cytosol
-
 Oryza sativa 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.1 additional information not affected by Na+, K+, Mg2+, Ca2+ Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.1 (1,4-alpha-D-glucosyl)n + phosphate Oryza sativa polysaccharide substrate is starch, phosphorolysis is the physiologic reaction of the starch degrading enzyme (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Oryza sativa
-
 cv. Tainong 67, enzyme Pho1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 from seedlings, 299fold to homogeneity by protamine sulfate and ammonium sulfate fractionation, anion exchange chromatography, and dextrin affinity chromatography Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 seedling etiolated, low enzyme content Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 additional information
-
 substrate specificity Oryza sativa
2.4.1.1 1.8
-
 purified enzyme Oryza sativa

Storage Stability

EC Number Storage Stability Organism
2.4.1.1 purified enzyme in 50 mM imidazole, pH 7.0, 1 mM EDTA, 1 mM DTT, at 4°C, at least 2 weeks stable Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 (1,4-alpha-D-glucosyl)n + phosphate polysaccharide substrate is starch, phosphorolysis is the physiologic reaction of the starch degrading enzyme Oryza sativa (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 (1,4-alpha-D-glucosyl)n + phosphate polysaccharide substrates are soluble starch and Zulkowsky soluble starch, amylopectin, glycogen, and dextrin, amyylopectin is the best substrate Oryza sativa (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 amylopectin + phosphate
-
 Oryza sativa amylopectin + D-glucose-1-phosphate
-
 ?
2.4.1.1 dextrin + alpha-D-glucose 1-phosphate
-
 Oryza sativa dextrin + phosphate
-
 ?
2.4.1.1 glycogen + phosphate
-
 Oryza sativa alpha-D-glucan + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 maltodecaose + alpha-D-glucose 1-phosphate low activity Oryza sativa ? + phosphate
-
 r
2.4.1.1 maltoheptaose + alpha-D-glucose 1-phosphate low activity Oryza sativa maltooctaose + phosphate
-
 r
2.4.1.1 maltohexaose + alpha-D-glucose 1-phosphate low activity Oryza sativa maltoheptaose + phosphate
-
 r
2.4.1.1 maltononaose + alpha-D-glucose 1-phosphate low activity Oryza sativa maltodecaose + phosphate
-
 r
2.4.1.1 maltooctaose + alpha-D-glucose 1-phosphate low activity Oryza sativa maltononaose + phosphate
-
 r
2.4.1.1 maltopentaose + alpha-D-glucose 1-phosphate
-
 Oryza sativa maltohexaose + phosphate
-
 r
2.4.1.1 maltotetraose + alpha-D-glucose 1-phosphate
-
 Oryza sativa maltopentaose + phosphate
-
 r
2.4.1.1 additional information substrate specificity, no or nearly no activity with glucose, maltose, and maltotriose, overview Oryza sativa ?
-
 ?
2.4.1.1 soluble starch + phosphate
-
 Oryza sativa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.1 alpha-glucan phosphorylase
-
 Oryza sativa
2.4.1.1 PHO
-
 Oryza sativa
2.4.1.1 SP
-
 Oryza sativa
2.4.1.1 starch phosphorylase
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 45 50
-
 Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 5
-
 synthesis reaction Oryza sativa
2.4.1.1 6
-
 assay at, phosphorolysis reaction Oryza sativa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.1 7
-
 most stable at Oryza sativa