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Literature summary extracted from

  • Ni, Y.; Chen, R.R.
    Lipoprotein mutation accelerates substrate permeability-limited toluene dioxygenase-catalyzed reaction (2005), Biotechnol. Prog., 21, 799-805.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.11 expressed in Escherichia coli Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.11 E609L increased activity with all substrates Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.11 toluene + NADH + O2 Pseudomonas putida
-
 (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.11 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.11 1-indanone + NADH + O2
-
 Pseudomonas putida (S)-2-hydroxy-1-indanone + NAD+
-
 ?
1.14.12.11 ethylbenzene + NADH + H+ + O2
-
 Pseudomonas putida cis-2,3-dihydroxy-2,3-dihydroethylbenzene + NAD+
-
 ?
1.14.12.11 toluene + NADH + O2
-
 Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.11 More part of a multiple enzyme complex: reductase, ferredoxin, dioxygenase Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.11 NADH
-
 Pseudomonas putida