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Literature summary extracted from

  • Munir, E.; Hattori, T.; Shimada, M.
    Purification and characterization of malate synthase from the glucose-grown wood-rotting basidiomycete Fomitopsis palustris (2002), Biosci. Biotechnol. Biochem., 66, 576-581.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.9 acetyl-CoA above 0.02 mM Fomitopsis palustris
2.3.3.9 ADP 1 mM, 50% residual activity Fomitopsis palustris
2.3.3.9 AMP 1 mM, 54% residual activity Fomitopsis palustris
2.3.3.9 ATP 1 mM, 50% residual activity Fomitopsis palustris
2.3.3.9 Ca2+ 5 mM, 19% residual activity Fomitopsis palustris
2.3.3.9 citrate 1 mM, 82% residual activity Fomitopsis palustris
2.3.3.9 coenzyme A 0.1 mM, 56% residual activity Fomitopsis palustris
2.3.3.9 D-fructose-1,6-bisphosphate 10 mM, 60% residual activity Fomitopsis palustris
2.3.3.9 glycolate 1 mM, 77% residual activity Fomitopsis palustris
2.3.3.9 Glyoxal 10 mM, 70% residual activity Fomitopsis palustris
2.3.3.9 iodoacetate 5 mM, 6% residual activity Fomitopsis palustris
2.3.3.9 K+ 5 mM, 5% residual activity Fomitopsis palustris
2.3.3.9 Mn2+ 5 mM, 16% residual activity Fomitopsis palustris
2.3.3.9 Na+ 5 mM, 10% residual activity Fomitopsis palustris
2.3.3.9 oxalate 1 mM, 43% residual activity Fomitopsis palustris
2.3.3.9 oxaloacetate 1 mM, 92% residual activity Fomitopsis palustris
2.3.3.9 p-chloromercuribenzoate 0.05 mM, 9% residual activity Fomitopsis palustris
2.3.3.9 phosphoenolpyruvate 1 mM, 66% residual activity Fomitopsis palustris
2.3.3.9 pyruvate 1 mM, 89% residual activity Fomitopsis palustris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.9 0.0022
-
 acetyl-CoA pH 8.0, 30°C Fomitopsis palustris
2.3.3.9 0.045
-
 glyoxalate pH 8.0, 30°C Fomitopsis palustris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.9 Mg2+ maximal enzyme activity at 5-10 mM, Km-value 0.4 mM Fomitopsis palustris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.9 65000
-
 8 * 65000, SDS-PAGE Fomitopsis palustris
2.3.3.9 520000
-
 gel filtration Fomitopsis palustris

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.9 Fomitopsis palustris
-
 growth on glucose
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.9
-
 Fomitopsis palustris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.9 acetyl-CoA + glyoxylate + H2O
-
 Fomitopsis palustris (S)-malate + CoA
-
 ?
2.3.3.9 additional information no substrate: oxaloacetate, pyruvate, 2-oxoglutarate, glyoxal Fomitopsis palustris ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.9 octamer 8 * 65000, SDS-PAGE Fomitopsis palustris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.9 8.5
-
-
 Fomitopsis palustris