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Literature summary extracted from

  • Matsuyama, K.; Matsumoto, H.; Matsuda, A.; Sugiura, H.; Komatsu, K.; Ichikawa, S.
    Purification of acetyl coenzyme A: deacetylacephalosporin C O-acetyltransferase from Acremonium chrysogenum (1992), Biosci. Biotechnol. Biochem., 56, 1410-1412.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.175 0.29
-
 deacetylcephalosporin C
-
 Acremonium chrysogenum
2.3.1.175 1.05
-
 acetyl-CoA
-
 Acremonium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.175 55000
-
 gel filtration Acremonium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.175 Acremonium chrysogenum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.175 proteolytic modification DCPC-ATF is synthesized as a single-chain precursor protein and is then converted to the two constituent subunit DCPC-ATF by proteolytic processing Acremonium chrysogenum

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.175
-
 Acremonium chrysogenum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.175 1.3
-
-
 Acremonium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.175 acetyl-CoA + deacetylcephalosporin C
-
 Acremonium chrysogenum CoA + cephalosporin C
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.175 ? SDS-PAGE resolves two protein bands with molecular weights of 27000 Da and 14000 Da Acremonium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
2.3.1.175 acetyl coenzyme A: deacetylacephalosporin C O-acetyltransferase
-
 Acremonium chrysogenum
2.3.1.175 DCPC-ATF
-
 Acremonium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.175 7.5
-
-
 Acremonium chrysogenum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.175 6 8.5 pH 6.0: about 60% of maximal activity, pH 8.5: about 50% of maximal activity Acremonium chrysogenum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.175 Acremonium chrysogenum chromatofocusing
-
 4