EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.9 | expression of wild-type and mutant enzymes in strain BL21(DE3) | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.9 | F2A | nearly inactive mutant, comparison of kinetics for wild-type and mutant enzymes | Escherichia coli |
2.5.1.9 | H102Q | highly reduced activity compared to the wild-type enzyme, comparison of kinetics for wild-type and mutant enzymes | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.9 | additional information | - |
additional information | steady-state and presteady-state kinetic analysis, single turnover conditions, transient stopped flow and quenched flow kinetics measurements with the pentacyclic reaction intermediate as substrate | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.9 | 6,7-dimethyl-8-(1-D-ribityl)lumazine | Escherichia coli | - |
riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.9 | Escherichia coli | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.5.1.9 | 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | pentacyclic reaction intermediate | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.9 | 6,7-dimethyl-8-(1-D-ribityl)lumazine | - |
Escherichia coli | riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione | - |
r | |
2.5.1.9 | 6,7-dimethyl-8-(1-D-ribityl)lumazine | mechanistically complex dismutation | Escherichia coli | riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione | - |
r | |
2.5.1.9 | additional information | utilization of the pentacyclic reaction intermediate as substrate | Escherichia coli | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.9 | trimer | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.9 | 25 | - |
assay at | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.9 | 0.001 | - |
6,7-dimethyl-8-(1-D-ribityl)lumazine | pH 6.9, 25°C, recombinant mutant H102Q, steady-state conditions | Escherichia coli | |
2.5.1.9 | 0.267 | - |
6,7-dimethyl-8-(1-D-ribityl)lumazine | pH 6.9, 25°C, recombinant wild-type enzyme, steady-state conditions | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.9 | 6.9 | - |
assay at | Escherichia coli |