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Literature summary extracted from

  • Bencurova, M.; Rendic, D.; Fabini, G.; Kopecky, E.M.; Altmann, F.; Wilson, I.B.H.
    Expression of eukaryotic glycosyltransferases in the yeast Pichia pastoris (2003), Biochimie, 85, 413-422.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.133 expression in Pichia pastoris Drosophila melanogaster
2.4.1.133 expression in Pichia pastoris Caenorhabditis elegans
2.4.2.26 inducible expression in Pichia pastoris Drosophila melanogaster
2.4.2.38 expression of N-terminally His5-tagged enzyme in Pichia pastoris, recombinant enzyme is secreted to the medium Arabidopsis thaliana

General Stability

EC Number General Stability Organism
2.4.2.38 the purified recombinant enzyme is not stable to a single freeze-thaw cycle Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.38 Mn2+
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.38 60000
-
 x * 60000, recombinant enzyme, SDS-PAGE Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.133 Caenorhabditis elegans
-
-
-
2.4.1.133 Drosophila melanogaster
-
-
-
2.4.2.26 Drosophila melanogaster
-
-
-
2.4.2.38 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.38 recombinant N-terminally His5-tagged enzyme from Pichia pastoris cell medium by nickel chelating affinity chromatography and ultrafiltration to homogeneity Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.38 0.33
-
 purified recombinant enzyme Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
2.4.2.38 4°C, purified recombinant enzyme, 3 weeks stable without loss of activity Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.38 UDP-xylose + dansylated Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,6)-(Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,3))-Manbeta-(1,4)-GlcNAcbeta-(1,4)-GlcNAc i.e. dansylated GnGn-peptide Arabidopsis thaliana UDP + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.38 ? x * 60000, recombinant enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.4.1.133 beta1,4-galactosyltransferase VII
-
 Drosophila melanogaster
2.4.1.133 beta1,4-galactosyltransferase VII
-
 Caenorhabditis elegans
2.4.1.133 SQV-3
-
 Drosophila melanogaster
2.4.1.133 SQV-3
-
 Caenorhabditis elegans
2.4.2.26 peptide O-xylosyltransferase
-
 Drosophila melanogaster
2.4.2.38 beta1,2-xylosyltransferase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.38 16
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.38 6.5
-
 assay at Arabidopsis thaliana