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Literature summary extracted from

  • Lledo, B.; Martinez-Espinosa, R.M.; Marhuenda-Egea, F.C.; Bonete, M.J.
    Respiratory nitrate reductase from haloarchaeon Haloferax mediterranei: biochemical and genetic analysis (2004), Biochim. Biophys. Acta, 1674, 50-59.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.7.2 azide 1 mM, 68% loss of activity Haloferax mediterranei
1.7.7.2 cyanide 1 mM, 76% loss of activity Haloferax mediterranei
1.7.7.2 Dithionite 1 mM, 24% loss of activity Haloferax mediterranei
1.7.7.2 EDTA 1 mM, 22% loss of activity Haloferax mediterranei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.7.2 0.25
-
 methyl viologen
-
 Haloferax mediterranei
1.7.7.2 0.82
-
 nitrate
-
 Haloferax mediterranei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.7.2 membrane
-
 Haloferax mediterranei 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.7.2 Iron enzyme contains Fe-S clusters. NarB codes for a 219-amino-acid-residue iron Rieske protein. Haloferax mediterranei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.7.2 additional information Haloferax mediterranei the regulation of the nar gene occurs at transcriptional level induced by oxygen-limiting conditions and the presence of nitrate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.2 Haloferax mediterranei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.2
-
 Haloferax mediterranei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.7.2 additional information
-
-
 Haloferax mediterranei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.2 additional information the regulation of the nar gene occurs at transcriptional level induced by oxygen-limiting conditions and the presence of nitrate Haloferax mediterranei ?
-
 ?
1.7.7.2 nitrate + reduced methyl viologen reduced methyl viologen is the best electron donor. NADPH or NADH show only 2.4% of maximal activity, no activity with FADH2 Haloferax mediterranei nitrite + methyl viologen
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.7.2 respiratory nitrate reductase
-
 Haloferax mediterranei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.7.2 70
-
 at 3.4 M NaCl Haloferax mediterranei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.7.2 7.9
-
 at 40°C Haloferax mediterranei
1.7.7.2 8.2
-
 at 60°C Haloferax mediterranei