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Literature summary extracted from

  • Kerscher, S.; Grgic, L.; Garofano, A.; Brandt, U.
    Application of the yeast Yarrowia lipolytica as a model to analyse human pathogenic mutations in mitochondrial complex I (NADH:ubiquinone oxidoreductase) (2004), Biochim. Biophys. Acta, 1659, 197-205.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.2
-
 Yarrowia lipolytica

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.2 E107A mutation in the 49000 Da subunit, no effect on complex I contant, mutant enzyme displays no deamino-nicotinamide-adeninedinucleotide:N-decylubiquinone activity Yarrowia lipolytica
7.1.1.2 F87L mutation in the 49000 Da subunit, no effect on complex I content, deamino-nicotinamide-adeninedinucleotide:N-decylubiquinone activity is reduced to aboput 60% of the parental strain value. The KM-value for n-decylubiquinone and the I50 value for rotenone are noemal Yarrowia lipolytica
7.1.1.2 P232Q mutation in thge 49000 Da subunit. Complex I assembly is severly impaired in this mutant. Deamino-nicotinamide-adeninedinucleotide:N-decylubiquinone activity is less than 20% of the normal value Yarrowia lipolytica
7.1.1.2 R199W mutation in the 30000 Da subunit, no significant alterations in complex I content or activity can be abserved in isolated mitochondrial membranes Yarrowia lipolytica
7.1.1.2 R231E mutation in the 49000 Da subunit of the complex, mutation has no effect on complex I content. Its deamino-nicotinamide-adeninedinucleotide:N-decylubiquinone activity is slightly reduced and its Km-value is somewhat elevated Yarrowia lipolytica
7.1.1.2 R231Q mutation in the 49000 Da subunit of the complex, no difference to wild-type enzyme in activity and stability Yarrowia lipolytica
7.1.1.2 S416A mutation in the 49000 Da subunit of the complex, mutation has no effect on complex I content. Its deamino-nicotinamide-adeninedinucleotide:N-decylubiquinone activity is slightly reduced. KM-value and I50 value for rotenone are both significantly higher than in the parental strain Yarrowia lipolytica
7.1.1.2 S416P mutation in the 49000 Da subunit of the complex, no difference to wild-type enzyme in activity and stability Yarrowia lipolytica
7.1.1.2 T157I mutation in the 30000 Da subunit, no significant alterations in complex I content or activity can be abserved in isolated mitochondrial membranes Yarrowia lipolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.2 2-decyl-4-quinazolinyl amine
-
 Yarrowia lipolytica
7.1.1.2 rotenone
-
 Yarrowia lipolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.2 0.007
-
 n-decylubiquinone mutant P232G (49000 Da subunit) Yarrowia lipolytica
7.1.1.2 0.012
-
 n-decylubiquinone mutant T157I (30000 Da subunit) Yarrowia lipolytica
7.1.1.2 0.014
-
 n-decylubiquinone mutant R211W (30000 Da subunit) Yarrowia lipolytica
7.1.1.2 0.016
-
 n-decylubiquinone mutant D228Q (49000 Da subunit) Yarrowia lipolytica
7.1.1.2 0.017
-
 n-decylubiquinone mutant S416P (49000 Da subunit) Yarrowia lipolytica
7.1.1.2 0.021
-
 n-decylubiquinone mutant F87L (49000 Da subunit) Yarrowia lipolytica
7.1.1.2 0.029
-
 n-decylubiquinone mutant R231E (49000 Da subunit) Yarrowia lipolytica
7.1.1.2 0.039
-
 n-decylubiquinone mutant S416A (49000 Da subunit) Yarrowia lipolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Yarrowia lipolytica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 deamino-nicotinamide-adeninedinucleotide + n-decylubiquinone
-
 Yarrowia lipolytica ? + n-decylubiquinol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 complex I
-
 Yarrowia lipolytica
7.1.1.2 NADH:ubiquinone oxidoreductase
-
 Yarrowia lipolytica