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Literature summary extracted from

  • Darnet, S.; Rahier, A.
    Enzymological properties of sterol-C4-methyl-oxidase of yeast sterol biosynthesis (2003), Biochim. Biophys. Acta, 1633, 106-117.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.9 6-amino-2-n-pentylbenzothiazole
-
Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.9 additional information
-
additional information kinetics Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.9 membrane
-
Saccharomyces cerevisiae 16020
-
1.14.18.9 microsome
-
Saccharomyces cerevisiae
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.9 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 Saccharomyces cerevisiae step 3 of 3 of the overall reaction 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
-
?
1.14.18.9 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 Saccharomyces cerevisiae FL530 step 3 of 3 of the overall reaction 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
-
?
1.14.18.9 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 Saccharomyces cerevisiae step 1 of 3 of the overall reaction 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
-
?
1.14.18.9 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 Saccharomyces cerevisiae FL530 step 1 of 3 of the overall reaction 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
-
?
1.14.18.9 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 Saccharomyces cerevisiae step 2 of 3 of the overall reaction 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
-
?
1.14.18.9 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 Saccharomyces cerevisiae FL530 step 2 of 3 of the overall reaction 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
-
?
1.14.18.9 additional information Saccharomyces cerevisiae enzyme plays a key role in sterol biosynthesis as component of the sterol-4-demethylation multienzyme complex, sterol metabolites analysis, overview ?
-
?
1.14.18.9 additional information Saccharomyces cerevisiae FL530 enzyme plays a key role in sterol biosynthesis as component of the sterol-4-demethylation multienzyme complex, sterol metabolites analysis, overview ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.9 Saccharomyces cerevisiae
-
enzyme is a component of the sterol-4-demethylation multienzyme complex
-
1.14.18.9 Saccharomyces cerevisiae FL530
-
enzyme is a component of the sterol-4-demethylation multienzyme complex
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.9 additional information
-
development of an enzyme assay for specific measurement of enzyme activity using [14C]-4,4-dimethyl-zymosterol as substrate Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.9 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 step 3 of 3 of the overall reaction Saccharomyces cerevisiae 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
-
?
1.14.18.9 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 step 3 of 3 of the overall reaction Saccharomyces cerevisiae FL530 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
-
?
1.14.18.9 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 step 1 of 3 of the overall reaction Saccharomyces cerevisiae 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
-
?
1.14.18.9 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 step 1 of 3 of the overall reaction Saccharomyces cerevisiae FL530 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
-
?
1.14.18.9 4,4-dimethyl-zymosterol + reduced acceptor + O2 artificial substrate Saccharomyces cerevisiae ?
-
?
1.14.18.9 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 step 2 of 3 of the overall reaction Saccharomyces cerevisiae 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
-
?
1.14.18.9 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 step 2 of 3 of the overall reaction Saccharomyces cerevisiae FL530 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
-
?
1.14.18.9 additional information enzyme plays a key role in sterol biosynthesis as component of the sterol-4-demethylation multienzyme complex, sterol metabolites analysis, overview Saccharomyces cerevisiae ?
-
?
1.14.18.9 additional information substrate specificity, 24-methylene-24-dihydrocycloartenol is a poor substrate, no activity with 24-ethylidene-24-dihydrolophenol, overview Saccharomyces cerevisiae ?
-
?
1.14.18.9 additional information enzyme plays a key role in sterol biosynthesis as component of the sterol-4-demethylation multienzyme complex, sterol metabolites analysis, overview Saccharomyces cerevisiae FL530 ?
-
?
1.14.18.9 additional information substrate specificity, 24-methylene-24-dihydrocycloartenol is a poor substrate, no activity with 24-ethylidene-24-dihydrolophenol, overview Saccharomyces cerevisiae FL530 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.14.18.9 More enzyme is a component of the sterol-4-demethylation multienzyme complex Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
1.14.18.9 4,4-dimethyl-sterol 4alpha-methyl-oxidase
-
Saccharomyces cerevisiae
1.14.18.9 4,4-dimethyl-zymosterol 4alpha-methyl-oxidase
-
Saccharomyces cerevisiae
1.14.18.9 SMO
-
Saccharomyces cerevisiae
1.14.18.9 sterol-C4-methyl-oxidase
-
Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.9 NADPH
-
Saccharomyces cerevisiae