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Literature summary extracted from

  • Sokolova, E.A.; Mirgorodskaya, O.A.; Roepstorff, P.; Savelyeva, N.V.; Zamolodchikova, T.S.
    Comparative study of the action of bovine duodenal proteinases (duodenases) on polypeptide substrates (2001), Biochemistry (Moscow), 66, 62-67.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B3 0.027
-
 angiotensinogen-(1-14) pH 8.9, 37°C Bos taurus
3.4.21.B3 0.03
-
 angiotensinogen-(1-14) pH 8.0, 37°C Bos taurus
3.4.21.B3 0.042
-
 angiotensinogen-(1-14) pH 8.9, 37°C Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.B3 polypeptide + H2O Bos taurus
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B3 Bos taurus P80219
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.21.B3 proteolytic cleavage of polypeptides to large and stable peptides, preferential cleavage sites: Lys-, Arg-, Tyr-, Phe-, Leu-, -Pro serine protease which has a dual specificity, i.e. both trypsin-like and chymotrypsin-like activities Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B3 alpha-N-succinyl-Val-Pro-Phe-4-nitroanilide + H2O
-
 Bos taurus alpha-N-succinyl-Val-Pro-Phe + 4-nitroaniline
-
 ?
3.4.21.B3 alpha-N-tosyl-Gly-Pro-Lys-4-nitroanilide + H2O
-
 Bos taurus alpha-N-tosyl-Gly-Pro-Lys + 4-nitroaniline
-
 ?
3.4.21.B3 angiotensinogen-(1-14) + H2O
-
 Bos taurus fragments of angiotensinogen-(1-14)
-
 ?
3.4.21.B3 glucagon + H2O
-
 Bos taurus fragments of glucagon
-
 ?
3.4.21.B3 human proinsulin + H2O cleaved bonds, overview Bos taurus fragments of human proinsulin
-
 ?
3.4.21.B3 melittin + H2O
-
 Bos taurus fragments of melittin
-
 ?
3.4.21.B3 polypeptide + H2O
-
 Bos taurus peptides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B3 ChlD
-
 Bos taurus
3.4.21.B3 DSD
-
 Bos taurus
3.4.21.B3 dual-specificity duodenase
-
 Bos taurus
3.4.21.B3 duodenase I
-
 Bos taurus
3.4.21.B3 duodenase serine protease
-
 Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.B3 33
-
 alpha-N-succinyl-Val-Pro-Phe-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 126
-
 angiotensinogen-(1-14) pH 8.9, 37°C Bos taurus
3.4.21.B3 168
-
 alpha-N-succinyl-Val-Pro-Phe-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 186
-
 angiotensinogen-(1-14) pH 8.0, 37°C Bos taurus