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Literature summary extracted from

  • Gladysheva, I.P.; Zamolodchikova, T.S.; Sokolova, E.A.; Larionova, N.I.
    Interaction between duodenase, a proteinase with dual specificity, and soybean inhibitors of Bowman-Birk and Kunitz type (1999), Biochemistry (Moscow), 64, 1244-1249.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B3 Bowman Birk soybean inhibitor the enzyme binds to the antichymotrypsin site of the inhibitor Bos taurus
3.4.21.B3 Kunitz type soybean trypsin inhibitor
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.B3 polypeptide + H2O Bos taurus
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B3 Bos taurus P80219
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.21.B3 proteolytic cleavage of polypeptides to large and stable peptides, preferential cleavage sites: Lys-, Arg-, Tyr-, Phe-, Leu-, -Pro belongs to the group of Janus-faced proteinases with dual specificity Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.B3 duodenum
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B3 alpha-N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Bos taurus alpha-N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
3.4.21.B3 alpha-N-succinyl-Val-Pro-Phe-4-nitroanilide + H2O
-
 Bos taurus alpha-N-succinyl-Val-Pro-Phe + 4-nitroaniline
-
 ?
3.4.21.B3 polypeptide + H2O
-
 Bos taurus peptides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B3 duodenase I
-
 Bos taurus
3.4.21.B3 duodenase serine protease
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B3 25
-
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B3 8
-
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.B3 0.0000027 0.000004 Bowman Birk soybean inhibitor pH 8.0, 25°C Bos taurus
3.4.21.B3 0.00004
-
 Kunitz type soybean trypsin inhibitor pH 8.0, 25°C Bos taurus
3.4.21.B3 0.0004
-
 Bowman Birk soybean inhibitor-trypsin complex pH 8.0, 25°C Bos taurus