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Literature summary extracted from

  • Lei, B.; Wang, H.; Yu, Y.; Tu, S.C.
    Redox potential and equilibria in the reductive half-reaction of Vibrio harveyi NADPH-FMN oxidoreductase (2005), Biochemistry, 44, 261-267.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.30 expression of wild-type enzyme and mutant R203A in Escherichia coli Vibrio harveyi
1.5.1.30 expression of wild-type enzyme in Escherichia coli Aliivibrio fischeri

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.30 R203A site-directed mutagenesis, no reversibility of the reduction of FMN, the half-reaction Vibrio harveyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.30 additional information
-
 additional information substrate and cofactor binding, reaction kinetics Aliivibrio fischeri
1.5.1.30 additional information
-
 additional information substrate and cofactor binding, reaction kinetics Vibrio harveyi

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.30 Aliivibrio fischeri
-
-
-
1.5.1.30 Vibrio harveyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.30 recombinant enzyme from Escherichia coli to homogeneity Aliivibrio fischeri
1.5.1.30 recombinant wild-type and mutant enzyme from Escherichia coli to homogeneity Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.30 riboflavin + NAD(P)H redox potential of the irreversible reductive half-reaction Aliivibrio fischeri reduced riboflavin + NADP+
-
 ?
1.5.1.30 riboflavin + NADPH redox potential and equilibria in the reversible reductive half-reaction Vibrio harveyi reduced riboflavin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.30 dimer
-
 Aliivibrio fischeri
1.5.1.30 dimer
-
 Vibrio harveyi

Synonyms

EC Number Synonyms Comment Organism
1.5.1.30 FRP
-
 Aliivibrio fischeri
1.5.1.30 FRP
-
 Vibrio harveyi
1.5.1.30 More see also EC 1.5.1.29 Aliivibrio fischeri
1.5.1.30 More see also EC 1.5.1.29 Vibrio harveyi
1.5.1.30 NADPH-FMN oxidoreductase
-
 Vibrio harveyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.30 7
-
 assay at Aliivibrio fischeri
1.5.1.30 7
-
 assay at Vibrio harveyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.30 FMN 1 FMN bound per subunit of the dimeric enzyme Aliivibrio fischeri
1.5.1.30 FMN 1 FMN bound per subunit of the dimeric enzyme Vibrio harveyi
1.5.1.30 NAD(P)H
-
 Aliivibrio fischeri
1.5.1.30 NADPH
-
 Vibrio harveyi