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Literature summary extracted from

  • Gunsior, M.; Ravel, J.; Challis, G.L.; Townsend, C.A.
    Engineering p-hydroxyphenylpyruvate dioxygenase to a p-hydroxymandelate synthase and Evidence for the proposed benzene oxide intermediate in homogentisate formation (2004), Biochemistry, 43, 663-674.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.27 ascorbate required for activity Streptomyces avermitilis
1.13.11.27 catalase from bovine liver, required for activity Streptomyces avermitilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.27 expression in Escherichia coli Streptomyces avermitilis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.27 F337I 0.67 of wild-type activity Streptomyces avermitilis
1.13.11.27 F341Y 16.9% of wild-type activity Streptomyces avermitilis
1.13.11.27 N216I 0.15% of wild-type activity Streptomyces avermitilis
1.13.11.27 N216I/F337I inactive Streptomyces avermitilis
1.13.11.27 N216I/F337I/F341Y inactive Streptomyces avermitilis
1.13.11.27 N216I/F341Y inactive Streptomyces avermitilis
1.13.11.27 P214T 1.6% of wild-type activity Streptomyces avermitilis
1.13.11.27 P214T/F337I/F341Y inactive Streptomyces avermitilis
1.13.11.27 P214T/N216I inactive Streptomyces avermitilis
1.13.11.27 P214T/N216I/F337I inactive Streptomyces avermitilis
1.13.11.27 P214T/N216I/F337I/F341Y inactive Streptomyces avermitilis
1.13.11.27 P214T/N216I/F341Y inactive Streptomyces avermitilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.27 Fe2+ required for activity Streptomyces avermitilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2 Streptomyces avermitilis
-
 homogentisate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.27 Streptomyces avermitilis Q53586
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2
-
 Streptomyces avermitilis homogentisate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.27 HPD
-
 Streptomyces avermitilis