EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.135 | expression of His-tagged truncated wild-type enzyme and His-tagged mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.135 | K104A | site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
2.3.1.135 | K133A | site-directed mutagenesis, increased activity compared to the truncated wild-type enzyme | Homo sapiens |
2.3.1.135 | K133A/K134A | site-directed mutagenesis, slightly increased activity compared to the truncated wild-type enzyme | Homo sapiens |
2.3.1.135 | K134A | site-directed mutagenesis, increased activity compared to the truncated wild-type enzyme | Homo sapiens |
2.3.1.135 | K147A | site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
2.3.1.135 | K180A | site-directed mutagenesis, nearly inactive mutant | Homo sapiens |
2.3.1.135 | K180R | site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
2.3.1.135 | K186A | site-directed mutagenesis, nearly inactive mutant | Homo sapiens |
2.3.1.135 | K186R | site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
2.3.1.135 | K90A | site-directed mutagenesis, highly reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
2.3.1.135 | K95A | site-directed mutagenesis, highly reduced activity compared to the truncated wild-type enzyme | Homo sapiens |
2.3.1.135 | Y118F | site-directed mutagenesis, activity similar to the truncated wild-type enzyme | Homo sapiens |
2.3.1.135 | Y154F | site-directed mutagenesis, inactive mutant | Homo sapiens |
2.3.1.135 | Y167F | site-directed mutagenesis, activity similar to the truncated wild-type enzyme | Homo sapiens |
2.3.1.135 | Y64F | site-directed mutagenesis, highly increased activity compared to the truncated wild-type enzyme | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.135 | phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] | Homo sapiens | - |
2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein] | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.135 | Homo sapiens | O95237 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.135 | recombinant His-tagged truncated wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography in presence of 1% SDS | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.135 | phosphatidylcholine + retinol-[cellular-retinol-binding-protein] = 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein] | mechanism, Cys161 and Tyr154 are essential for activity | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.135 | additional information | - |
activity of recombinant mutant enzymes and recombinant truncated wild-type, overview | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.135 | phosphatidylcholine + all-trans-retinol | transfer of acyl moiety from sn-1 position of lecithin, i.e. phosphatidylcholine, to vitamin A, i.e. all-trans-retinol | Homo sapiens | 2-acylglycerophosphocholine + all-trans-retinyl acylester | - |
? | |
2.3.1.135 | phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] | - |
Homo sapiens | 2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein] | - |
? | |
2.3.1.135 | phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] | transfer of acyl moiety from sn-1 position of lecithin, i.e. phosphatidylcholine, to vitamin A, i.e. all-trans-retinol | Homo sapiens | 2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein] | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.135 | lecithin-retinol acyltransferase | - |
Homo sapiens |
2.3.1.135 | LRAT | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.135 | 22 | - |
assay at room temperature | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.135 | 9 | - |
about | Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.135 | additional information | - |
pH profile of recombinant mutant enzymes and recombinant truncated wild-type | Homo sapiens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.135 | 10.4 | - |
inactivation of recombinant truncated wild-type enzyme, reversible by dialysis against a buffer with pH 8.4 | Homo sapiens |