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Literature summary extracted from

  • Xue, L.; Rando, R.R.
    Roles of cysteine 161 and tyrosine 154 in the lecithin-retinol acyltransferase mechanism (2004), Biochemistry, 43, 6120-6126.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.135 expression of His-tagged truncated wild-type enzyme and His-tagged mutant enzymes in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.135 K104A site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme Homo sapiens
2.3.1.135 K133A site-directed mutagenesis, increased activity compared to the truncated wild-type enzyme Homo sapiens
2.3.1.135 K133A/K134A site-directed mutagenesis, slightly increased activity compared to the truncated wild-type enzyme Homo sapiens
2.3.1.135 K134A site-directed mutagenesis, increased activity compared to the truncated wild-type enzyme Homo sapiens
2.3.1.135 K147A site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme Homo sapiens
2.3.1.135 K180A site-directed mutagenesis, nearly inactive mutant Homo sapiens
2.3.1.135 K180R site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme Homo sapiens
2.3.1.135 K186A site-directed mutagenesis, nearly inactive mutant Homo sapiens
2.3.1.135 K186R site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme Homo sapiens
2.3.1.135 K90A site-directed mutagenesis, highly reduced activity compared to the truncated wild-type enzyme Homo sapiens
2.3.1.135 K95A site-directed mutagenesis, highly reduced activity compared to the truncated wild-type enzyme Homo sapiens
2.3.1.135 Y118F site-directed mutagenesis, activity similar to the truncated wild-type enzyme Homo sapiens
2.3.1.135 Y154F site-directed mutagenesis, inactive mutant Homo sapiens
2.3.1.135 Y167F site-directed mutagenesis, activity similar to the truncated wild-type enzyme Homo sapiens
2.3.1.135 Y64F site-directed mutagenesis, highly increased activity compared to the truncated wild-type enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.135 phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] Homo sapiens
-
2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein]
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.135 Homo sapiens O95237
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.135 recombinant His-tagged truncated wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography in presence of 1% SDS Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.135 phosphatidylcholine + retinol-[cellular-retinol-binding-protein] = 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein] mechanism, Cys161 and Tyr154 are essential for activity Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.135 additional information
-
activity of recombinant mutant enzymes and recombinant truncated wild-type, overview Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.135 phosphatidylcholine + all-trans-retinol transfer of acyl moiety from sn-1 position of lecithin, i.e. phosphatidylcholine, to vitamin A, i.e. all-trans-retinol Homo sapiens 2-acylglycerophosphocholine + all-trans-retinyl acylester
-
?
2.3.1.135 phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein]
-
Homo sapiens 2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein]
-
?
2.3.1.135 phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] transfer of acyl moiety from sn-1 position of lecithin, i.e. phosphatidylcholine, to vitamin A, i.e. all-trans-retinol Homo sapiens 2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein]
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.135 lecithin-retinol acyltransferase
-
Homo sapiens
2.3.1.135 LRAT
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.135 22
-
assay at room temperature Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.135 9
-
about Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.135 additional information
-
pH profile of recombinant mutant enzymes and recombinant truncated wild-type Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.135 10.4
-
inactivation of recombinant truncated wild-type enzyme, reversible by dialysis against a buffer with pH 8.4 Homo sapiens