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Literature summary extracted from

  • Draker, K.A.; Wright, G.D.
    Molecular mechanism of the enterococcal aminoglycoside 6'-N-acetyltransferase': role of GNAT-conserved residues in the chemistry of antibiotic inactivation (2004), Biochemistry, 43, 446-454.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.82 E72A mutation causes a notable reduction in affinity for both the 4,5- and 4,5-disubstituted classes of aminoglycosides, with the largest changes being an 83fold decrease in the affinity for the minimal substrate neamine and a 175fold reduction in Km-value for kanamycin Enterococcus faecium
2.3.1.82 H74A kinetic parameters reveal only a small effect of the mutation on enzyme activity, Km-values for acetyl-CoA and the 4,5- and 4,6-disubstituted aminoglycosides only differs by a range of 2-7.5fold compared to that of the wild-type enzyme, indicating no significant change in apparent affinity Enterococcus faecium
2.3.1.82 L76A mutant enzyme shows virtually wild-type activity, with the biggest changes being a 3.6fold reduction in the apparent affinity for neomycin and a 3.7fold decrease in turnover number for amikacin Enterococcus faecium
2.3.1.82 L76P mutant enzyme is impaired in both aminoglycoside recognition and catalysis, 2300fold decrease in the ratio of turnover number to Kb-value for neamine, 1300fold reduction in the ratio of turnover number to Kb-value for tobramycin, no detectable activity towardskanamycin A and amikacin Enterococcus faecium
2.3.1.82 Y147A no detectable activity towardsamikacin Enterococcus faecium
2.3.1.82 Y147F decrease in aminoglycoside affinity ranges from a 6fold decrease for tobramycin to a large 170fold change in KM-value for amikacin Enterococcus faecium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.82 0.0033
-
acetyl-CoA pH 6.0, 37°C, mutant enzyme L76A Enterococcus faecium
2.3.1.82 0.0041
-
acetyl-CoA pH 6.0, 37°C, mutant enzyme Y147F Enterococcus faecium
2.3.1.82 0.0047
-
acetyl-CoA pH 6.0, 37°C, mutant enzyme Y147A Enterococcus faecium
2.3.1.82 0.0053
-
neomycin pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
2.3.1.82 0.0058
-
neamine pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
2.3.1.82 0.0073
-
acetyl-CoA pH 6.0, 37°C, mutant enzyme H74A Enterococcus faecium
2.3.1.82 0.0091
-
ribostamycin pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
2.3.1.82 0.0114
-
amikacin pH 6.0, 37°C, mutant enzyme L76A Enterococcus faecium
2.3.1.82 0.0131
-
amikacin pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
2.3.1.82 0.0144
-
kanamycin A pH 6.0, 37°C, mutant enzyme H74A Enterococcus faecium
2.3.1.82 0.0182
-
neomycin pH 6.0, 37°C, mutant enzyme H74A Enterococcus faecium
2.3.1.82 0.019
-
neomycin pH 6.0, 37°C, mutant enzyme L76A Enterococcus faecium
2.3.1.82 0.0199
-
kanamycin A pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
2.3.1.82 0.0214
-
ribostamycin pH 6.0, 37°C, mutant enzyme L76A Enterococcus faecium
2.3.1.82 0.022
-
tobramycin pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
2.3.1.82 0.0224
-
neamine pH 6.0, 37°C, mutant enzyme L76A Enterococcus faecium
2.3.1.82 0.0235
-
acetyl-CoA pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
2.3.1.82 0.0257
-
kanamycin A pH 6.0, 37°C, mutant enzyme L76A Enterococcus faecium
2.3.1.82 0.029
-
neomycin pH 6.0, 37°C, mutant enzyme E72A Enterococcus faecium
2.3.1.82 0.0369
-
tobramycin pH 6.0, 37°C, mutant enzyme H74A Enterococcus faecium
2.3.1.82 0.0381
-
ribostamycin pH 6.0, 37°C, mutant enzyme H74A Enterococcus faecium
2.3.1.82 0.0386
-
neomycin pH 6.0, 37°C, mutant enzyme Y147A Enterococcus faecium
2.3.1.82 0.0432
-
neamine pH 6.0, 37°C, mutant enzyme H74A Enterococcus faecium
2.3.1.82 0.0489
-
acetyl-CoA pH 6.0, 37°C, mutant enzyme L76P Enterococcus faecium
2.3.1.82 0.0553
-
neomycin pH 6.0, 37°C, mutant enzyme Y147F Enterococcus faecium
2.3.1.82 0.0573
-
neomycin pH 6.0, 37°C, mutant enzyme L76P Enterococcus faecium
2.3.1.82 0.0582
-
amikacin pH 6.0, 37°C, mutant enzyme H74A Enterococcus faecium
2.3.1.82 0.0682
-
neamine pH 6.0, 37°C, mutant enzyme L76P Enterococcus faecium
2.3.1.82 0.107
-
ribostamycin pH 6.0, 37°C, mutant enzyme L76P Enterococcus faecium
2.3.1.82 0.109
-
ribostamycin pH 6.0, 37°C, mutant enzyme Y147A Enterococcus faecium
2.3.1.82 0.11
-
kanamycin A pH 6.0, 37°C, mutant enzyme Y147A Enterococcus faecium
2.3.1.82 0.136
-
tobramycin pH 6.0, 37°C, mutant enzyme Y147F Enterococcus faecium
2.3.1.82 0.146
-
tobramycin pH 6.0, 37°C, mutant enzyme L76P Enterococcus faecium
2.3.1.82 0.147
-
tobramycin pH 6.0, 37°C, mutant enzyme Y147A Enterococcus faecium
2.3.1.82 0.15
-
neamine pH 6.0, 37°C, mutant enzyme Y147F Enterococcus faecium
2.3.1.82 0.154
-
acetyl-CoA pH 6.0, 37°C, mutant enzyme E72A Enterococcus faecium
2.3.1.82 0.169
-
neamine pH 6.0, 37°C, mutant enzyme Y147A Enterococcus faecium
2.3.1.82 0.301
-
ribostamycin pH 6.0, 37°C, mutant enzyme E72A Enterococcus faecium
2.3.1.82 0.306
-
ribostamycin pH 6.0, 37°C, mutant enzyme Y147F Enterococcus faecium
2.3.1.82 0.34
-
kanamycin A pH 6.0, 37°C, mutant enzyme Y147F Enterococcus faecium
2.3.1.82 0.38
-
amikacin pH 6.0, 37°C, mutant enzyme E72A Enterococcus faecium
2.3.1.82 0.482
-
neamine pH 6.0, 37°C, mutant enzyme E72A Enterococcus faecium
2.3.1.82 0.62
-
tobramycin pH 6.0, 37°C, mutant enzyme L76A Enterococcus faecium
2.3.1.82 1.15
-
tobramycin pH 6.0, 37°C, mutant enzyme E72A Enterococcus faecium
2.3.1.82 2.19
-
amikacin pH 6.0, 37°C, mutant enzyme Y147F Enterococcus faecium
2.3.1.82 3.49
-
kanamycin A pH 6.0, 37°C, mutant enzyme E72A Enterococcus faecium

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.82 Enterococcus faecium
-
chromosomally encoded aminoglycoside 6'-N-acetyltransferase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.82
-
Enterococcus faecium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.82 acetyl-CoA + amikacin
-
Enterococcus faecium CoA + N6'-acetylamikacin
-
?
2.3.1.82 acetyl-CoA + kanamycin A
-
Enterococcus faecium CoA + N6'-acetylkanamycin A
-
?
2.3.1.82 acetyl-CoA + neamine
-
Enterococcus faecium CoA + N6'-acetylneamine
-
?
2.3.1.82 acetyl-CoA + neomycin
-
Enterococcus faecium CoA + N6'-acetylneomycin
-
?
2.3.1.82 acetyl-CoA + ribostamycin
-
Enterococcus faecium CoA + N6'-acetylribostamycin
-
?
2.3.1.82 acetyl-CoA + tobramycin
-
Enterococcus faecium CoA + N6'-acetyltobramycin
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.82 aminoglycoside 6'-N-acetyltransferase
-
Enterococcus faecium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.82 0.001
-
neomycin pH 6.0, 37°C, mutant enzyme Y147A Enterococcus faecium
2.3.1.82 0.002
-
neamine pH 6.0, 37°C, mutant enzyme L76P Enterococcus faecium
2.3.1.82 0.003
-
amikacin pH 6.0, 37°C, mutant enzyme Y147F Enterococcus faecium
2.3.1.82 0.004
-
tobramycin pH 6.0, 37°C, mutant enzyme Y147A Enterococcus faecium
2.3.1.82 0.004
-
neamine pH 6.0, 37°C, mutant enzyme Y147A Enterococcus faecium
2.3.1.82 0.01
-
tobramycin pH 6.0, 37°C, mutant enzyme L76P Enterococcus faecium
2.3.1.82 0.01
-
ribostamycin pH 6.0, 37°C, mutant enzyme L76P Enterococcus faecium
2.3.1.82 0.01
-
acetyl-CoA pH 6.0, 37°C, mutant enzyme Y147A Enterococcus faecium
2.3.1.82 0.01
-
kanamycin A pH 6.0, 37°C, mutant enzyme Y147A Enterococcus faecium
2.3.1.82 0.02
-
ribostamycin pH 6.0, 37°C, mutant enzyme Y147A Enterococcus faecium
2.3.1.82 0.03
-
amikacin pH 6.0, 37°C, mutant enzyme L76A Enterococcus faecium
2.3.1.82 0.03
-
kanamycin A pH 6.0, 37°C, mutant enzyme Y147F Enterococcus faecium
2.3.1.82 0.04
-
amikacin pH 6.0, 37°C, mutant enzyme H74A Enterococcus faecium
2.3.1.82 0.04
-
neomycin pH 6.0, 37°C, mutant enzyme L76P Enterococcus faecium
2.3.1.82 0.05
-
amikacin pH 6.0, 37°C, mutant enzyme E72A Enterococcus faecium
2.3.1.82 0.05
-
kanamycin A pH 6.0, 37°C, mutant enzyme H74A Enterococcus faecium
2.3.1.82 0.05
-
neamine pH 6.0, 37°C, mutant enzyme Y147F Enterococcus faecium
2.3.1.82 0.06
-
acetyl-CoA pH 6.0, 37°C, mutant enzyme L76P Enterococcus faecium
2.3.1.82 0.07
-
acetyl-CoA pH 6.0, 37°C, mutant enzyme Y147F Enterococcus faecium
2.3.1.82 0.07
-
neomycin pH 6.0, 37°C, mutant enzyme Y147F Enterococcus faecium
2.3.1.82 0.1
-
tobramycin pH 6.0, 37°C, mutant enzyme Y147F Enterococcus faecium
2.3.1.82 0.11
-
amikacin pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
2.3.1.82 0.12
-
ribostamycin pH 6.0, 37°C, mutant enzyme Y147F Enterococcus faecium
2.3.1.82 0.15
-
neamine pH 6.0, 37°C, mutant enzyme H74A Enterococcus faecium
2.3.1.82 0.15
-
ribostamycin pH 6.0, 37°C, mutant enzyme L76A Enterococcus faecium
2.3.1.82 0.16
-
neomycin pH 6.0, 37°C, mutant enzyme L76A Enterococcus faecium
2.3.1.82 0.18
-
acetyl-CoA pH 6.0, 37°C, mutant enzyme H74A Enterococcus faecium
2.3.1.82 0.19
-
neomycin pH 6.0, 37°C, mutant enzyme H74A Enterococcus faecium
2.3.1.82 0.2
-
neomycin pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
2.3.1.82 0.23
-
ribostamycin pH 6.0, 37°C, mutant enzyme H74A Enterococcus faecium
2.3.1.82 0.23
-
acetyl-CoA pH 6.0, 37°C, mutant enzyme L76A Enterococcus faecium
2.3.1.82 0.27
-
kanamycin A pH 6.0, 37°C, mutant enzyme L76A Enterococcus faecium
2.3.1.82 0.3
-
tobramycin pH 6.0, 37°C, mutant enzyme H74A Enterococcus faecium
2.3.1.82 0.34
-
ribostamycin pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
2.3.1.82 0.35
-
ribostamycin pH 6.0, 37°C, mutant enzyme E72A Enterococcus faecium
2.3.1.82 0.38
-
neamine pH 6.0, 37°C, mutant enzyme L76A Enterococcus faecium
2.3.1.82 0.4
-
acetyl-CoA pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
2.3.1.82 0.42
-
neamine pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
2.3.1.82 0.44
-
neamine pH 6.0, 37°C, mutant enzyme E72A Enterococcus faecium
2.3.1.82 0.55
-
kanamycin A pH 6.0, 37°C, mutant enzyme E72A Enterococcus faecium
2.3.1.82 0.82
-
kanamycin A pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
2.3.1.82 0.89
-
tobramycin pH 6.0, 37°C, mutant enzyme L76A Enterococcus faecium
2.3.1.82 0.99
-
acetyl-CoA pH 6.0, 37°C, mutant enzyme E72A Enterococcus faecium
2.3.1.82 1.1
-
tobramycin pH 6.0, 37°C, wild-type enzyme Enterococcus faecium
2.3.1.82 1.1
-
tobramycin pH 6.0, 37°C, mutant enzyme E72A Enterococcus faecium
2.3.1.82 1.1
-
neomycin pH 6.0, 37°C, mutant enzyme E72A Enterococcus faecium