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Literature summary extracted from

  • Purdy, M.M.; Koo, L.S.; Ortiz de Montellano, P.R.; Klinman, J.P.
    Steady-state kinetic investigation of cytochrome P450cam: interaction with redox partners and reaction with molecular oxygen (2004), Biochemistry, 43, 271-281.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.1 putidaredoxin
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.1 additional information
-
 additional information Km-value of putidaredoxin, 0.0038 mM, steady-state kinetics of enzyme interaction with redox partners Pseudomonas putida
1.14.15.1 0.077
-
 O2 recombinant enzyme with His6-tag, pH 7.4, 25°C Pseudomonas putida
1.14.15.1 0.083
-
 O2 wild-type, pH 7.4, 25°C Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.1 (+)-camphor + reduced putidaredoxin + O2 = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O putidaredoxin acts as a shuttle for transport of electrons from putidaredoxin reductase to enzyme Pseudomonas putida
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + putidaredoxin + O2
-
 Pseudomonas putida (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.15.1 55
-
 O2 recombinant enzyme with His6-tag, pH 7.4, 25°C Pseudomonas putida
1.14.15.1 66
-
 O2 wild-type, pH 7.4, 25°C Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 cytochrome P450
-
 Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.15.1 0.00038
-
 putidaredoxin recombinant enzyme with His6-tag, pH 7.4, 25°C Pseudomonas putida
1.14.15.1 0.0007
-
 putidaredoxin wild-type, pH 7.4, 25°C Pseudomonas putida